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Anupam, obviously you haven't been here for a while... Charles offered him a whiskey.Anupam Basu wrote:...They look alive - so how are you getting them under a microscope and once there - to stay still enough for focus stacks. Thanks - I'd appreciate any insights.
-anupam
Thanks for the clarification. I tend to do in the field photography of live critters and even when they are still in the morning, have found it very difficult to do more than a few shots at a reasonably high magnification with reversed enlarger lenses, so I was wondering if there's a trick I am missing.Charles Krebs wrote:Anupam... Welcome!
Unfortunately, with a compound microscope, if you intend to do a "stack" for extended DOF, your subjects need to be perfectly still. This usually means they are either anesthetized (a rather inexact science!), or recently deceased (very exact... but unfortunate). Usually, if I resort to the latter I will do the overall shots first, and then progress to higher magnification images of tiny parts and details, perhaps even making mounted slides.