Bosmina longirostris, 2 images added

Images made through a microscope. All subject types.

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rekuwi
Posts: 83
Joined: Mon Jan 12, 2009 9:22 am
Location: Wiesbaden, Deutschland

Bosmina longirostris, 2 images added

Post by rekuwi »

Hallo,

in the "Hattsteinweiher" near Usingen lives this waterflea. I show You fife Images in different focal planes:

Image

Image

Image

Image

Image

Objektiv 25 x, DIK.

Best regards
Regi
Last edited by rekuwi on Wed Aug 08, 2012 1:27 pm, edited 1 time in total.

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Very nice Cladoceran. One I've never seen myself. Looks from your different focal plane images as though they might stack to give you a single image with nice depth of focus. I don't know if you have tried that? Also looks like you have a green colour cast issue and a bit of purple fringing. Both of these are easily fixed in Photoshop or other image processing software to give you a nice grey background .
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

rekuwi
Posts: 83
Joined: Mon Jan 12, 2009 9:22 am
Location: Wiesbaden, Deutschland

Post by rekuwi »

Hallo Dave,

thank You for answering.
The Cladoceran mowed his legs. So stacking was not possible.
Surely I could made the background grey, it's easy with photoshop. In order to remove color fringing Unfortunately I lack the knowledge in this program. But I'll try to learn.

Best regards
Regi

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

He looks like a little elephant. Nice images. :)

rekuwi
Posts: 83
Joined: Mon Jan 12, 2009 9:22 am
Location: Wiesbaden, Deutschland

Bosmina longirostris

Post by rekuwi »

Hallo Dave,

one image with grey background:

Image

one without any colour:

Image

Do You think that's better?

Hallo Mitch,

I think so too :)

best regards
Regi

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Personally I do prefer the grey background. It's easy to remove that blue/purple fringing as well. In Photoshop go to the Enhance tab. Select Adjust Colour from the first drop down and Adjust Hue/Saturation from the new drop down. In the Adjust Hue/Saturation window replace the default choice of Edit Master with Edit Blues from the drop down. Place the Eyedropper symbol over a typical area of blue fringing, you may need to zoom in to do this accurately. Then move the Saturation slider to the left watching the effect as you do so. about -80% usually does the job. Watch that other colours are not being affected too much.
Once you are happy with the result it is usually worth applying a little sharpening and increasing contrast a little.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

rekuwi
Posts: 83
Joined: Mon Jan 12, 2009 9:22 am
Location: Wiesbaden, Deutschland

Bosmina

Post by rekuwi »

Thank you Dave for the description. I've tried it and this is the result:

Image

best regards
Regi

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

What do you think? I think that came out pretty well.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

rekuwi
Posts: 83
Joined: Mon Jan 12, 2009 9:22 am
Location: Wiesbaden, Deutschland

Post by rekuwi »

Dave, I like it too!
Nice that I now know how I can eliminate color fringing. (It's really easy).


:D
Regi

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