Yeast DE-DIC revisited

curt0909 · Post by **curt0909** » Wed Aug 01, 2012 2:08 am

I finally broke ground on a project that I had put on the back burner for the last year and it was somewhat of a success. I sacrificed some perfectly good legos and a cheap microscope to create a nosepiece that allows vertical, horizontal and angluar adjustments of a DIC combining prism. By manipulating the upper prism I am able to increase or decrease the contrast effect to some degree. I've also added a third prism underneath the condenser(not pictured) which further increases contrast.
http://www.youtube.com/watch?v=BHfoLFiY ... e=youtu.be
http://www.youtube.com/watch?v=YWAsSUqH ... e=youtu.be

Jacek · Post by **Jacek** » Wed Aug 01, 2012 7:50 am

amazing design, the effect came out really good, I peel admiration for you

Pau · Post by **Pau** » Wed Aug 01, 2012 8:36 am

Interesting DIY setup! Nice work putting all that parts working together.

RogelioMoreno · Post by **RogelioMoreno** » Wed Aug 01, 2012 9:55 am

Very good results with your DIY setup.

Rogelio

curt0909 · Post by **curt0909** » Wed Aug 01, 2012 1:37 pm

Thanks for the comments.

The simplicity and versatility of this makes me wonder why vertical and angular manipulation of the combining prism wasn't more common on finite scopes. It would greatly increase the number of objectives usable with the DIC system. On modern scopes it would not be as useful as most are infinity corrected. In this case vertical prism manipulation would not have any effect on the contrast. I suspect angular adjustments should still have an effect though. I will test it with my reichert infinity objectives.