Yeast DE-DIC revisited

Images made through a microscope. All subject types.

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curt0909
Posts: 609
Joined: Wed Oct 26, 2011 10:06 am
Location: Pittsburgh, PA

Yeast DE-DIC revisited

Post by curt0909 »

I finally broke ground on a project that I had put on the back burner for the last year and it was somewhat of a success. I sacrificed some perfectly good legos and a cheap microscope to create a nosepiece that allows vertical, horizontal and angluar adjustments of a DIC combining prism. By manipulating the upper prism I am able to increase or decrease the contrast effect to some degree. I've also added a third prism underneath the condenser(not pictured) which further increases contrast.
http://www.youtube.com/watch?v=BHfoLFiY ... e=youtu.be
http://www.youtube.com/watch?v=YWAsSUqH ... e=youtu.be

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Jacek
Posts: 5357
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

amazing design, the effect came out really good, I peel admiration for you

Pau
Site Admin
Posts: 6105
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Interesting DIY setup! Nice work putting all that parts working together.
Pau

RogelioMoreno
Posts: 2979
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Very good results with your DIY setup.

Rogelio

curt0909
Posts: 609
Joined: Wed Oct 26, 2011 10:06 am
Location: Pittsburgh, PA

Post by curt0909 »

Thanks for the comments.

The simplicity and versatility of this makes me wonder why vertical and angular manipulation of the combining prism wasn't more common on finite scopes. It would greatly increase the number of objectives usable with the DIC system. On modern scopes it would not be as useful as most are infinity corrected. In this case vertical prism manipulation would not have any effect on the contrast. I suspect angular adjustments should still have an effect though. I will test it with my reichert infinity objectives.

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