Chlorophyta, Elodea cyclosis, Paramecium (photo added)

Images made through a microscope. All subject types.

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curt0909
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Chlorophyta, Elodea cyclosis, Paramecium (photo added)

Post by curt0909 »

Video on youtube best seen in HD 720p. Nomarski DIC. Nikon 60x 1.40 Planapo. ID of Chlorophyta would be greatly appreciated. Specimen from North East USA http://www.youtube.com/watch?v=z3iqoczT ... ature=plcp

Here is a still of cytoplasmic streaming in Elodea
Image

BJ
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Post by BJ »

hi Curt,

another nice video with excellent detail.

I think that your green alga is a non-motile cell of a Chlamydomonas species.

A single basal pyrenoid (great to see the surrounding starch grains so clearly) and a cup-shaped chloroplast put it in the subgenus Euchlamydomonas.

The cell shape and particularly the lack of a protuberance at the apex suggest it may be Chlamydomonas reinhardtii - one of the commonest species in the genus and definitely found in puddles and small ponds.

I often find it difficult to identify organisms from a single photo. With your video and the changes in focus through the cell it is much easier.

thank you for sharing

brian

RogelioMoreno
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Post by RogelioMoreno »

Very nice video!

Rogelio

Charles Krebs
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Post by Charles Krebs »

Curt,

The 720p video looks great.

At the end of the video there are some ciliates and a rotifer where the lighting appears to be somewhat uneven... the middle looks brighter than the edges. Is that how it appears through the eyepieces?

Litonotus
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Post by Litonotus »

great video (: the image is also very good.
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

curt0909
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Post by curt0909 »

Brian, thank you for your identification. Your knowledge on the topic is impressive. I prefer to shoot video for that reason. It can provide much more information than stills.

Rogelio, Charles, Litonotus thank you.

Charles, with certain objectives the DIC prisms do not make a good match. I try to hid this by adding a gradient to the opposite side in post.

Reichert Diastar infinity microscope with 1.40 NA DIC condenser
I have 10, 20, 40 reichert plan achromats and a 100x reichert plan fluor 1.30 that give a nice even background DIC image, a 40 reichert neoplan that shows a strong gradient, nikon 20, 40, 60, 100 planapos(noninfinity) that give mixed results. The 60 is a great match. Even when using 30mm ultrawide eyepieces the background is relatively even. The 40 is not a good combination but it gives acceptable DIC if I stop down the condenser. here's an example. The first 2 scenes are done with the nikon 40x plan apo. http://www.youtube.com/watch?v=eeuONqXD ... ature=plcp

arturoag75
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Post by arturoag75 »

Superb job :shock: :wink:
arturo

curt0909
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Post by curt0909 »

Thanks Art :D

fpelectronica
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Post by fpelectronica »

Good video!
Francisco

Jacek
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Post by Jacek »

Beautiful photo and video

curt0909
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Post by curt0909 »

Thank you Francisco and Jacek

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