Panacea prola ("Prola Beauty") wing section

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Panacea prola ("Prola Beauty") wing section

Post by Charles Krebs »

Quite a striking and beautiful butterfly overall. This particular wing section (dorsal forewing) was particularly fascinating to me because of the variety of scales and hairs present in a very small area.

(Been away for close to two months working on a project that occupied nearly every waking hour... zero time for any "personal" work. Peeked in occasionally and have just looked over the postings during this period. Wow! Some really great work being posted. I've been here since about "start-up" for this forum and I don't think there's ever been a period where such interesting and excellent microscope work has been regularly found here. =D> to all!)


Olympus 5/0.15 UPLFL, Olympus U-TLU tube lens, Nikon MM-11 microscope, Canon T3i. 5X on sensor
Image

USMC 10/0.28 M Plan Apo, Olympus U-TLU tube lens, Nikon MM-11 microscope, Canon T3i. 9X on sensor.
Image

Jacek
Posts: 5360
Joined: Sun Oct 02, 2011 7:00 am
Location: Poland

Post by Jacek »

great :shock: :shock: :shock:

Rylee Isitt
Posts: 476
Joined: Fri Apr 13, 2012 3:54 pm
Location: Canada
Contact:

Post by Rylee Isitt »

These wing sections are excellent abstract textural photography. You really should print these at 11x17" or larger and put them on your wall :)

arturoag75
Posts: 1600
Joined: Fri Feb 05, 2010 1:05 am
Location: italy
Contact:

Post by arturoag75 »

Superb shot Charlie! :lol:
arturo

naturephoto1
Posts: 509
Joined: Sun Nov 13, 2011 5:37 pm
Location: Breinigsville, PA
Contact:

Post by naturephoto1 »

Welcome back Charlie. Outstanding images.

Rich

RogelioMoreno
Posts: 2982
Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Wow, super shots! :shock:

It is like being there!

Rogelio
Last edited by RogelioMoreno on Sun Jun 24, 2012 12:29 pm, edited 1 time in total.

ChrisR
Site Admin
Posts: 8671
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

Welcome back Charles, fine images again.
Looking back at a couple of attempts of mine at this sort of subject, I either have to diffuse like crazy or have trouble with highlights. Underexposure is often needed but darks block before lights are clear of burn-out.
You've managed shodows, with consequent nice relief, without the highlights going into orbit.
Is there anything like 16 bit processing, or HDR you're using, which helps with that?

hayath
Posts: 301
Joined: Tue Apr 03, 2012 6:18 am
Location: Bangalore, India
Contact:

Post by hayath »

Wowsome!
Some fine detail and great color reproduction :)

pwnell
Posts: 2031
Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Great work as always. This is not as easy as it looks - getting nice light is VERY difficult. Too soft and you lose the iridescence. Too harsh and you get specular highlights. Well done!

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Thanks all!

Chris
I either have to diffuse like crazy or have trouble with highlights. Underexposure is often needed but darks block before lights are clear of burn-out.
You've managed shodows, with consequent nice relief, without the highlights going into orbit.
Is there anything like 16 bit processing, or HDR you're using, which helps with that?
These are stacked from regular out-of camera 8-bit/channel jpgs, with a slight "twist" or two. Because of the variety of scales.. some colored only by pigment, others (the blues ones) with significant iridescence it did require a bit more fiddling with the light than normal. This was on a "vertical" apparatus, and a tall "cylinder" of Lee diffusion material was placed around the subject on the stage (2-3 inches in diameter, open at the top, unlike the domes I sometimes use). Three of the Ikea lights were used. The "main light" was very close to the diffusion material and slightly above the wing level. This provided a smaller light-source (but still pretty diffuse) than if the light were positioned farther away. That, plus the low angle gave nice relief (sense of depth). But as you mentioned, then the shadows risk going too black. So the other two Ikea lights were used to provide sufficient fill light without destroying the main lighting effect. Moving them closer and farther away, trying different angles while observing the effect and shooting test shots eventually determined the best position of those lights. I couldn't avoid the bright wing vein with the lighting (even tried a single polarizer on the lens) so I shot an additional stack of the wing vein section at a darker exposure in case I wanted to use that later on. The full image was stacked both Pmax and Dmap. The bulk of the image is Pmax (especially the parts with the "hairs"). The top left scales looked better in Dmap so that was used there. The bright veins were pretty bad in Pmax, but looked better Dmap so that was used there as well. (So I didn't end up incorporating the darker exposure for this version.) In all stacks the blue scales were just a bit too bright, so they were darkened via a second layer that had some levels and curves done to it.

The key really was the ability to change the lighting look with those flexible Ikea lights while observing the effect. Moving them in right up against the diffuser give a much different look than when they are pulled back a few inches. Slight angle shifts make a very noticeable difference. It's also useful to play around with the diameter and height of the diffusion "cylinder" and how close or far it is placed to the subject.

ChrisR
Site Admin
Posts: 8671
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

Thanks for the detailed explanation.
Sounds like the point where I need flash to avoid vibration problems (about 2x) is where the control of the Jansjo lights becomes more useful. I should be able to do it with flash(es), - not controlling it finely enough at the mo.

SteveGreen1953
Posts: 44
Joined: Thu Mar 22, 2012 11:26 am
Location: Sycamore, IL USA

Post by SteveGreen1953 »

Wonderful images. Nicely done! =D>

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Chris,

I recall your mentioning somewhere the overall "environmental" vibration issues you need to deal with. It sure would be nice to small flexible light sources that can be used with a continuous accurate modeling light for set-up purposes, but then expose via flash to deal with external vibration issues. I've recently experimented a little more (yet again!) with FO light guides and simple flash coupling. Would be doable, but my shooting environment is stable enough that I can use the Ikea lights with the EFSC and not have any difficulty in that regard. So there is no great compelling urgency to add yet another project to my... (already too long)... list! :wink:

ChrisR
Site Admin
Posts: 8671
Joined: Sat Mar 14, 2009 3:58 am
Location: Near London, UK

Post by ChrisR »

need to deal with
That's right. We'll be moving house, to get me a concrete floor!

I have a Schott FO illuminator. I've never been able to use it.

abpho
Posts: 1526
Joined: Wed Aug 17, 2011 7:11 pm
Location: Earth

Post by abpho »

Very groovy. That must be one big butterfly.

Post Reply Previous topicNext topic