Desmids (images added ++)

Images made through a microscope. All subject types.

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naturephoto1
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Post by naturephoto1 »

Rogelio,

Great images.

Rich

RogelioMoreno
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Post by RogelioMoreno »

Thank you all for your comments.

Rogelio

RogelioMoreno
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Post by RogelioMoreno »

descall wrote:Staggeringly wonderful!! :D :D
Could you describe the sharpening method you use please?
Many thanks,
Des
Try the following:
1. Apply Unsharp Mask with amount: 120%, radius: 1 and threshold: 3
2. Under Edit menu choose Fade Unsharp Mask and change the mode to Luminosity (leave the opacity to 100%) and click Ok.

Rogelio

Blcak
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Post by Blcak »

Amazing pictures, Rogelio.
I need to get myself a microscope one day. Haven't used one since my school days, and these particular hours were too short.
arturoag75 wrote:Rogelio please stop otherwise i jump out of windows whit my scope ...and my 600D :lol: :cry: :wink:
incredible shots!
just a question:
my canon does not give me pictures so bright and shiny like yours even if they are visually .... is there some trick? in addition to your skills and the microscope?? :?:
best
arturo
Have you or anyone else here ever tried the LAB mode in photoshop for photomicrography shots?
Dan Margulis has written a book about it, I'm still not done with it though.

However, increasing the colours with a curve in a/b channel and then sharpening only the L-channel produced some nice results already. I guess you'd have to be careful with the background, though.

arturoag75
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Post by arturoag75 »

..no ..what is LAB mode? :?:
can you tell me some steps?
best
Arturo

Blcak
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Post by Blcak »

arturoag75 wrote:..no ..what is LAB mode? :?:
can you tell me some steps?
best
Arturo
I'm not really competent (yet) with this mode, but I can give you some starting advice.

In Photoshop (don't know from which CS on), you can change the colour mode in a picture (RGB, CMYK, etc.). LAB is among that. To work in LAB mode, you first have to (or, should) open a file in 16 bit mode, then convert it to LAB (Image > Mode > LAB).

When you look at the channels (next to your layers), you'll find a, b, and lightness (I'll call it "l"). The first two contain your colours, the third contains the contrast. It's sort of like a monochrome picture combined with two colour layers. You can sharpen the "l" channel and tune the colours in a and b.

I'm experimenting with curves for colour channels, open a curves layer (or copy your image layer and press ctrl+m) and select a, then later b, then make the graph more narrow (move both ends inwards one square or so). The resulting layer will look "too much", but you can use the layer fill/opacity to adjust that. This is particulary useful for giving a dull picture more vivid colours. I've found problems with single toned areas, though, but those I use a mask for.

Sharpening is only done to the l-channel. So colours are not affected by the process.

Afterwards you of course change the mode to RGB/8bit and convert in sRGB colour space to save your JPG for web ;).

I might be able to add more once I've finished the book (which is to take quite a while with my schedule), but I also lack experience with this and that won't change for a while. I was just wondering if anyone here has found it useful in photomicrography since you're dealing with such particulary fine subjects :).

yanyading
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Post by yanyading »

GOOD! :shock:
The another good viewing method can beat DIC is dark field.
--
UV is the Key to High Resolution Microscopy

arturoag75
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Post by arturoag75 »

Blcak wrote:
arturoag75 wrote:..no ..what is LAB mode? :?:
can you tell me some steps?
best
Arturo
I'm not really competent (yet) with this mode, but I can give you some starting advice.

In Photoshop (don't know from which CS on), you can change the colour mode in a picture (RGB, CMYK, etc.). LAB is among that. To work in LAB mode, you first have to (or, should) open a file in 16 bit mode, then convert it to LAB (Image > Mode > LAB).

When you look at the channels (next to your layers), you'll find a, b, and lightness (I'll call it "l"). The first two contain your colours, the third contains the contrast. It's sort of like a monochrome picture combined with two colour layers. You can sharpen the "l" channel and tune the colours in a and b.

I'm experimenting with curves for colour channels, open a curves layer (or copy your image layer and press ctrl+m) and select a, then later b, then make the graph more narrow (move both ends inwards one square or so). The resulting layer will look "too much", but you can use the layer fill/opacity to adjust that. This is particulary useful for giving a dull picture more vivid colours. I've found problems with single toned areas, though, but those I use a mask for.

Sharpening is only done to the l-channel. So colours are not affected by the process.

Afterwards you of course change the mode to RGB/8bit and convert in sRGB colour space to save your JPG for web ;).

I might be able to add more once I've finished the book (which is to take quite a while with my schedule), but I also lack experience with this and that won't change for a while. I was just wondering if anyone here has found it useful in photomicrography since you're dealing with such particulary fine subjects :).
many thanks for you informations! :wink:
arturo

Mitch640
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Post by Mitch640 »

#3 would make an excellent print.

RogelioMoreno
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Post by RogelioMoreno »

Thank you all for your comments. :D

Mitch, I agree #3 would make a excellent print. :wink:

Rogelio

RogelioMoreno
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Post by RogelioMoreno »

Here a set of Micrasterias rotata with : DIC, DarkField and oblique illumination:

Image

Image

Image

Image

Image

Rogelio

arturoag75
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Post by arturoag75 »

:shock: :shock: :?
arturo

Litonotus
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Post by Litonotus »

superb, as usual. obligue light is interesting, I wonder how my universal filter would work on that great equipment...
my FB page

I'm looking for the the extemely rare V-IM magnification changer for the E800 scope. If you have seen a listing or have one for sale please let me know.

descall
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Post by descall »

Please excuse my ignorance, but I've never tried oblique lighting. I'm guessing it is the third image, which looks rather like some DIC I see! For the darkfield, what condenser set-up did you use? Best wishes, Des

naturephoto1
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Post by naturephoto1 »

Rogelio,

Outstanding additional set of photos.

Rich

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