Chewing Louse: Polarization vs. brightfield

Images made through a microscope. All subject types.

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NikonUser
Posts: 2676
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Chewing Louse: Polarization vs. brightfield

Post by NikonUser »

This literally fell out of the sky.
Two crows flew over my yard, one aggressively chasing the other; contact was made and 2 feathers floated down. This chewing louse was on one of the feathers.
It's in the Family: Philopteridae (Mallophaga:Ambycera). Length: 1.50 mm.
I thought I liked the effect on polarization with some arthropods but with this louse regular brightfield gives a more pleasing image.
Both with 4x Nikon objective on an Olympus BHS with 1.25x intermediate lens and a 2.5x NFK relay lens. Without and with polarizers engaged.
The row of dots right center edge is a feather 'filament' it appeared o be feeding on.
Image
NU12054
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

Ahh! One of my favourite subjects. :wink: I've greatly enjoyed imaging some old Victorian slides of chewing lice, they retained amazing muscle detail and looked well with polarisation and DIC as well as brightfield. www.photomacrography.net/forum/viewtopi ... ight=louse
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

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