Spores on the Wind-Part 1

Images made through a microscope. All subject types.

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Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Spores on the Wind-Part 1

Post by Walter Piorkowski »

Image

Image

Image

Spores on the Wind-Part 1

Leitz Ortholux
Transmitted light brightfield
Leitz 54x Fluorite objective oiled, NA 0.95
8x GF WFC projection eyepiece with 1/2x relay lens
Strobe illumination
Canon 10D
Photoshop



Like many people in the world I suffer certain times of the year from an allergic reaction to plant pollens in the air. Five years ago I set out to take a look at these pollens. I constructed an air sampling device which, unfortunately, failed completely to catch a single pollen.

It did however turn out to be remarkably efficient at collecting tens of thousands of mold spores. Along with these were insect parts, plant parts, even diatoms and algae. Also the microscopic garbage in the air including my winter favorite, wood ash from fireplaces and wood stoves still showing cellular structure!

I finally have a microscope set up well enough to do justice to the collections of my multi-year experiment. The spores exhibit a great variety of forms, color and size as well as how they get collected in my chamber. Most come in singly, while others always come in groups. Either way they are all entering our lungs and sticking to our eyes every day. So I would like to show you what they look like.

Finally I begin my atlas of the unidentified spores for all of you to enjoy. The next several submissions will be subjects captured in the summer between July and August.

On a technical note, you will notice a red cast to the background of the images that have lighter spores in them. The subjects were all illuminated with a strobe under the control of a C. Krebs controller with no filters in the optical path. Only a glass sheet type beam splitter. Maybe one you more experienced imagers can help me to diagnose this problem.

Walt

Charles Krebs
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Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
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Post by Charles Krebs »

Walter... great project! Very interesting! (Also...please describe your apparatus for "capturing" your subjects)

The red background is almost certainly the result of the microscope's incandescent "viewing" illumination being too close in intensity to the flash illumination. This issue is most noticeable in brightfield illumination where the microscope is quite efficient with light. As a result you are getting some "daylight" light from the flash, but also a large dose of "incandescent" light is registering in the image. This will very quickly give you a reddish color tonality.

Insure that you are using the fastest possible flash-sync speed on your camera body. Then reduce the incandescent illumination to the level where... if you were to take the shot without the flash "firing"... just about nothing is recorded... (the image looks nearly black). Then you should be fine.

If you shoot these in "raw" files it will be easy to adjust to the proper color temperature on conversion. But ideally, when using flash, you don't want the incandescent light to "register" an image at all. Especially true with moving subjects, since you risk getting a somewhat blurred "ghost" image from the incandescent along with a "sharp" image from the flash.

Frankly, with brightfield and static subjects I will sometimes use the incandescent light (with no flash) since it is so bright. I use the mirror lock-up, and shutter speeds are quite high... enough so that vibration is not an issue.

Walter Piorkowski
Posts: 693
Joined: Mon Aug 14, 2006 6:42 pm
Location: South Beloit, Ill

Post by Walter Piorkowski »

Thank you Charles, you are right on the money. I had the incandecent light source high to overcome the light loss due to the beam splitter. I would be happy to use the bulb for these non moving subjects but pushing even 7 volts through my 6 volt bulb doesn't seem to give as sharp and color pleasing illumination as the strob. I will have to find a way to provide halogen light someday.

Regarding my sampler, give me some time to get some images together for you.
Walt

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