Help ID, amoeba?

carlos.uruguay · Post by **carlos.uruguay** » Tue Apr 17, 2012 7:18 am

Link:
http://www.youtube.com/watch?v=vvGXqwDkgAU
About 23um of diameter
freshwater
40x and 100x objectives
Phase contrast oblique light and brightfield
Gocevia? Cochliopodium? Otherwise?
Thanks to all

Bruce Taylor · Post by **Bruce Taylor** » Tue Apr 17, 2012 9:05 am

Perhaps an organism in a cyst. In the early part, "focusing artifacts" mimic pseudopodia, but the final sequence (1:30 - end) seems to show a fixed membrane around the creature.

carlos.uruguay · Post by **carlos.uruguay** » Tue Apr 17, 2012 9:09 am

Hi Bruce.
is probably
I do not know
I think it could be a cyst of amoeba
carlos

Bruce Taylor · Post by **Bruce Taylor** » Tue Apr 17, 2012 9:11 am

Yes, maybe. Encysted ciliates & flagellates look quite amorphous & amoeboid, too.

Ferry · Post by **Ferry** » Tue Apr 17, 2012 2:28 pm

Cysts don't move. You will be surprised, it's a shelled amoeba

. To be more specific, I should know how it looks in side view, but I guess it's a Pyxidicula.

carlos.uruguay · Post by **carlos.uruguay** » Tue Apr 17, 2012 6:40 pm

Thanks Ferry.
There were many organisms, more than 20.
just below the coverslip
They seemed flat
At the beginning of the video with oblique light straight I thought I saw some straight limits on the edge
I will try to film more

Bruce Taylor · Post by **Bruce Taylor** » Tue Apr 17, 2012 8:20 pm

Yes, Ferry, thanks for the correction. I was thinking it might be moving because it was in an early stage of excystment.

Ecki · Post by **Ecki** » Wed Apr 18, 2012 1:42 am

You can see the contractile vacuole - cysts don't have one.

Bruce Taylor · Post by **Bruce Taylor** » Wed Apr 18, 2012 4:46 am

Ecki wrote:You can see the contractile vacuole - cysts don't have one.

Except while they are "awakening:

http://www.youtube.com/watch?v=kon-aP2DSyg

But yes, my suggestion was a poor one. I have more enthusiasm than experience.