The first is a stack of 18 photos under my 100x objective (oil immersed). It is very hard to stack at this magnification... Had to retouch by hand (used Helicon Focus). Uncropped photo. From what I understand those two spheres are oxygen bubbles formed by photosynthesis that the diatom uses for buoyancy.
Another diatom (I think), normal 40x objective and cropped. Single photo. Both diatoms from the ocean off Crescent Beach, BC, Canada:
