Prestomal teeth in the blowfly proboscis -- pictures added

Images made through a microscope. All subject types.

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Cactusdave
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Prestomal teeth in the blowfly proboscis -- pictures added

Post by Cactusdave »

In a thread in the 'Technical and Studio Photography' section, Rik has shown some beautiful stacks at high and low power of the blowfly proboscis in an intact fly. http://www.photomacrography.net/forum/v ... hp?t=16216 The level of detail shown using a X50 objective is superb and the structure of the (pre)stomal teeth and the pseudo-tracheae can be clearly seen. As Rik notes, this corresponds to the diagram shown on page 145 of B. Thompson Lowne's comprehensive and wonderful Victorian study of blowfly anatomy http://www.biodiversitylibrary.org/item ... 1/mode/1up

To get an idea of the kind of mounted material Lowne would have been working from, I dug out a contemporary slide of the blowfly tongue by noted Victorian slide mounter Charles Morgan Topping. I located the same area on the slide and and took a single image in the focal plane of the (pre)stomal teeth. The microscope is a Nikon Diaphot with LWD 0.52 phase/DIC condenser. The camera was a Canon 40D front mounted at the microscope SLR port and there is an in-microscope X2.5 magnification factor. The objective used was a Zeiss X25 0.65 planapochromat in conjunction with the Nikon X40 DIC prism.

Image

To put this focal plane in context I also made a short stack of 11 images, which place the (pre)stomal teeth better in relation to the pseudo-tracheae and this corresponds quite nicely with Lowne's illustration.

Image

For orientation of this area on the whole mount of the blowfly tongue, here is a large stitch of 100 or so images of the mount from which the previous two detailed images are taken. This was taken with a Nikon X10 0.25 Plan DIC objective using the same microscope setup last year. The area of the proboscis in question is roughly outlined by the red box.

Image

It is fascinating to see how much more information would have been available to Lowne if modern stacking techniques had been available to him. He would be astonished to see what is possible now, but equally it is remarkable what he achieved with the equipment available to him.
Last edited by Cactusdave on Wed Mar 07, 2012 4:35 am, edited 3 times in total.
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rjlittlefield
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Post by rjlittlefield »

Wonderful images -- many thanks for the follow-up.

--Rik

naturephoto1
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Post by naturephoto1 »

Dave,

Remarkable amount of detail in these new images.

Rich

Cactusdave
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Post by Cactusdave »

Thanks Rik. I had meant to post that big stitch of the whole blowfly mouth parts mount, a while back, but never got round to it. A bunch of interesting new slides came along, and I started off on another project.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Cactusdave
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Post by Cactusdave »

Thanks for your comment Rich. BTW I have edited the first post to correct a factual error that crept in. All three images, the two showing detail and the large overview stitch are from the same slide by Charles Morgan Topping.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

RogelioMoreno
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Post by RogelioMoreno »

Cactusdave,

Amazing details, specially the stacked image!

Rogelio

Cactusdave
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Post by Cactusdave »

Thanks Rogelio. The Zeiss X25 0.65 Planapo is a fine lens. I was able to afford it because it was honestly sold as having some delamination. In fact the delamination is very minor and hard to find. Also this lens works very well with the Nikon X40 DIC prism. I have tried many different non-DIC lenses with the Nikon DIC condenser on the Diaphot and while many give some DIC effects, usually in a rather narrow band, the Zeiss X25 gives an almost even field of good DIC contrast.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Cactusdave
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Post by Cactusdave »

Having seen Rik's new post and Nikonuser's beautiful image I thought I would have another look at my mounted preparation. I have taken a couple of small stitches (4-5 images) from the opposite side of the proboscis from my earlier images. I think the picture is a little clearer in this area. I think there are two sets of 'teeth' one which bifurcates to pass on either side of a psedotracheal opening, and a shorter set which are possibly more heavily sclerotized which project into the space between adjacent pseudotracheae.
These images wer both taken with the Zeiss X25 lens. The first one is ateempting to visualise the plane of the 'short teeth', though the bifurcating teeth can also be seen.

Image

The second image attempts to concentrate on thr focal plane of the bifurcating teeth.


Image


The third image is an 11 image stitch of the same area with a Nikon X40 0.65 Plan DIC objective.


Image
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Cactusdave
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Post by Cactusdave »

Final thought on this one. I found another slide of the blowfly proboscis in one of my boxes. This one is significantly more transparent so allows a slightly different view. The lens was a Lomo X30 0.90 water immersion with the built in iris stopped down about halfway. Stack of 29 images. The X40 DIC prism was in the light path.

Image

Two sets of 'teeth' are visible in this image as well as structures that might be supporting or strengthening the entrance to the pseudotracheae.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

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