Franz Neidl wrote:Hello Rogelio,
I am very much impressed by the quality of your diatom pictures! In order to better understand your method (and maybe to improve my pictures) I would need some exif-data, for exemple: shutter speed, exposer mode (manual?), live view, what kind of flash etc.
Congratulation!
Franz
The following is my normal configuration:
Shutter speed: 1/200
Expouser mode: Tv
Mirror lock up, no live view.
Nikon SB-80 flash in manual mode: with the 20x objective I normally shoot at 1/2 power or less (when the camera shows that I am clipping the highlight). With the 40x at full power. I have to look for a more powerful flash if I want to shoot with 60x (I do not have it) or 100x and the camera mounted on the front port of the TE300 (the front port has a 2x photo lens).
Actually I am using DIC system that is used on Nikon 80i/90i/Ti; but I did a test with DIC system that is used on E600/E800/E1000 and no differences in image quality were found.
One point to mention is that my Senarmont polarizer and analyzer are both high transmission, from the following link:
http://www.olympusmicro.com/primer/tech ... ation.html
I quote the following paragraph:
"The polarizers employed in DIC microscopy are similar or identical to those used for polarized light observations, but many manufacturers offer matched DIC polarizing filters that have both low and high light transmission efficiencies. At the upper end of the magnification range (40x, 60x, and 100x), polarizers with high transmission efficiencies are preferred for DIC because specimen details are more clearly delineated and larger bias retardation values can be utilized for video and digital imaging. The low transmission polarizing filters are useful for DIC observation using the 10x and 20x objectives, but severely restrict light transmission at higher magnifications."
Hope this help.
Thank you very much for your comments.
Rogelio
