Loxophyllum, Stylonychia and Naupliar

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 5:01 pm

Here recent images:

1 and 2 Loxophyllum.
3 Stylonychia (I think it is Stylonychia) with 4 macronuclei.
4 and 5 Naupliar

All with Nikon TE300 microscope, Plan Apo 20x/0.75 objective, DIC, flash and Canon T3i mounted on the TE300 front port (2x).

Let me know your comments.

Rogelio

naturephoto1 · Post by **naturephoto1** » Sat Feb 04, 2012 5:04 pm

Rogelio,

Stunning images and lighting. I particularly like the first three images and the first one has particularly nice orientation.

Rich

Mitch640 · Post by **Mitch640** » Sun Feb 05, 2012 5:06 am

Amazing images. I have never seen a Stylonychia in such detail. The polarizing effect even lights up some of the particles.

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 05, 2012 10:10 am

Rich and Mitch,

Thank you.

Rogelio

Tom Jones · Post by **Tom Jones** » Sun Feb 05, 2012 10:23 am

Rogelio,

Beautiful images!!

I'm presuming some of the reason you are able to get such stunning clarity across nearly the entire surface, is the fact that the organisms are more or less flat against the bottom of the dish. Since you are using DIC, plastic petri dishes are probably out. Are you using glass dishes, slides, or inverted slides with the coverslip on the bottom to eliminate the effect of the thick glass? I'm guessing inverted slides.

Tom

Cactusdave · Post by **Cactusdave** » Sun Feb 05, 2012 10:35 am

Super pictures, the resolution of the microfilaments in the first two is astonishing, really what we used to call 'research grade' in the lab.

Jacek · Post by **Jacek** » Sun Feb 05, 2012 10:46 am

Comment is superfluous, wonderful images, excellent

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 05, 2012 11:17 am

Tom Jones wrote:Rogelio,

Beautiful images!!

I'm presuming some of the reason you are able to get such stunning clarity across nearly the entire surface, is the fact that the organisms are more or less flat against the bottom of the dish. Since you are using DIC, plastic petri dishes are probably out. Are you using glass dishes, slides, or inverted slides with the coverslip on the bottom to eliminate the effect of the thick glass? I'm guessing inverted slides.

Tom

I am using coverslip for both sides, the stage on inverted move in both direction together with what you put on it (on upright the stage move in one direction together with the slide, the other direction only move the slide).

Thank you for your comments.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 05, 2012 11:19 am

Cactusdave wrote:Super pictures, the resolution of the microfilaments in the first two is astonishing, really what we used to call 'research grade' in the lab.

I am also amazed that I got that extrusomes so detailed, the kineties can be seen too.

Thank you for your comments.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 05, 2012 11:19 am

Jacek,

Thank you for your comments.

Rogelio