How is this try at bacteria?

pwnell · Post by **pwnell** » Fri Feb 03, 2012 4:02 pm

Last time I struggled with my Nikon E200 100x objective in oil. Same setup, just a fresh attempt. I had a brine shrimp that died in some water, and these bacteria started growing exponentially. Taken under the 100x oil objective under Phase Contrast.

Opinions?

Mitch640 · Post by **Mitch640** » Fri Feb 03, 2012 6:16 pm

Nice and sharp. How did you stop the motion using a 100x? Or maybe they weren't moving?

Charles Krebs · Post by **Charles Krebs** » Fri Feb 03, 2012 8:22 pm

Waldo,

This looks very good for phase contrast with a 100X. Bacteria of this type are a tough subject with a "light" microscope.

I think you could boost the contrast with a "levels" or "curves" adjustment, and add a little unsharp mask and it would look a bit better and not over-processed.

pwnell · Post by **pwnell** » Fri Feb 03, 2012 9:20 pm

Some of the bacteria were swimming about, but most of these were pretty static.

PS: I did process it in Photoshop - this is after levels adjustment, curves and sharpening, both a global and local sharpening mask were applied.

Jan l'Amie · Post by **Jan l'Amie** » Sat Feb 04, 2012 8:17 am

Nice pictures. I also made some bacteria photo's. I let them grow on a petri-dish with Agar, ented with some pondwater.
In the begin there was a lot of moving but after a few minuts they where all static. A Plan40/0.65 and 2.5 photo-oculair used. Kreutz filter for oblique light.