How is this try at bacteria?

Images made through a microscope. All subject types.

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pwnell
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How is this try at bacteria?

Post by pwnell »

Last time I struggled with my Nikon E200 100x objective in oil. Same setup, just a fresh attempt. I had a brine shrimp that died in some water, and these bacteria started growing exponentially. Taken under the 100x oil objective under Phase Contrast.

Opinions?
Image

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Nice and sharp. How did you stop the motion using a 100x? Or maybe they weren't moving?

Charles Krebs
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Post by Charles Krebs »

Waldo,

This looks very good for phase contrast with a 100X. Bacteria of this type are a tough subject with a "light" microscope.

I think you could boost the contrast with a "levels" or "curves" adjustment, and add a little unsharp mask and it would look a bit better and not over-processed.

pwnell
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Joined: Fri Dec 18, 2009 4:59 pm
Location: Tsawwassen, Canada

Post by pwnell »

Some of the bacteria were swimming about, but most of these were pretty static.

PS: I did process it in Photoshop - this is after levels adjustment, curves and sharpening, both a global and local sharpening mask were applied.

Jan l'Amie
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Post by Jan l'Amie »

Nice pictures. I also made some bacteria photo's. I let them grow on a petri-dish with Agar, ented with some pondwater.
In the begin there was a lot of moving but after a few minuts they where all static. A Plan40/0.65 and 2.5 photo-oculair used. Kreutz filter for oblique light.

Image

Image
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot
www.ngvm.nl

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