Acineta

RogelioMoreno · Post by **RogelioMoreno** » Fri Feb 03, 2012 4:59 am

Here a set of Acineta images.

All with Nikon TE300 microscope, Plan Apo 20x/0.75 objective, DIC, flash and Canon T3i mounted on the TE300 front port (2x).

Rogelio

Litonotus · Post by **Litonotus** » Fri Feb 03, 2012 5:09 am

the last two - no words.

Pau · Post by **Pau** » Fri Feb 03, 2012 8:53 am

Rogelio, Nice picture of stars collision, but be aware this is a microscopy forum and not an astrophysics one

The quality your are getting is just incredibile. If you don't stop posting this beauties I'm going to throw my poor DIC scope to the litter and moving to another hobby (joking again)

arturoag75 · Post by **arturoag75** » Fri Feb 03, 2012 1:05 pm

No words.....

just a question: do you shot in RAW format?..your pics are so clear and brilliant that i've dubt on my T3 camera...
is there some trick in post production??

best and congratulation for your job!
best
Arturo

Cactusdave · Post by **Cactusdave** » Fri Feb 03, 2012 1:52 pm

I'm with Pau on this one!

Looking for a really big skip to chuck my Diaphot into! Only joking!

Seriously I know now why I don't try and do more of this kind of photography and content myself mainly with prepared slides, I just can't get anywhere near the quality that Rogelio and the other DIC kings are achieving with live subjects. Someday I'll set that flash up, when I have a little more time, and a little more space ....... In the meanwhile congratulations again on some more extraordinary photos.

Charles Krebs · Post by **Charles Krebs** » Fri Feb 03, 2012 2:27 pm

Rogelio,

These are great!

Very talented user, great equipment and set-up.

More than any of the images you have posted recently I think these also show what I had wondered about earlier... the benefit of having gravity working for you instead of against you. With an inverted microscope you have situations where, due to gravity, multiple key parts of the subject are right up against the coverslip. (This appears to be really obvious in your third image). With an upright you would be lucky to have one or two of the tentacles... 1) in the same focal plane, and 2) against the cover slip for best optical quality. Using wet mounts in an upright, gravity tends to pull your subject away from the best location for optical quality. Often you will have a considerable layer of water between the cover and the subject... and this can really kill the image quality of a high NA objective due to increased spherical aberration. (With live material I think this can also lead to more "flattened" and distorted subjects when the photomicrographer tries to minimize this water layer).

Most inverted microscopes I have seen are set-up with very modest (NA) condensers and no DIC capability, so it is quite a revelation to see your wonderful images.

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 12:07 pm

Litonotus,

Thank you for your comments.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 12:09 pm

Pau wrote:Rogelio, Nice picture of stars collision, but be aware this is a microscopy forum and not an astrophysics one

The quality your are getting is just incredibile. If you don't stop posting this beauties I'm going to throw my poor DIC scope to the litter and moving to another hobby (joking again)

Pau, let me know if you trow it (to go and pick up it for me, joking).

Thank you very much for your kindly words.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 12:23 pm

arturoag75 wrote:No words.....
just a question: do you shot in RAW format?..your pics are so clear and brilliant that i've dubt on my T3 camera...
is there some trick in post production??
best and congratulation for your job!
best
Arturo

Arturo, yes I shot in RAW.

I always try to get that maximum dynamic range that I can, with my DIC setup you get maximum dynamic range at maximum extinction (I think that it is normal of all DIC system); but at maximum extinction the background is dark and some subjects look weird, you have to move a little away from maximum extinction to get a balanced image.

I normally do the following:
- Convert the RAW to tiff.
- Crop the image to get a closer look to the subject.
- Contrast adjustment.
- Color cast correction (when necesary).
- Use the spot healing tool to remove unwanted particules.
- Sharpening.
- Noise reduction.
- Save the tiff.
- Resize to half (or less) and save as jpg.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 12:24 pm

Cactusdave,

Thank you for your comments.

Rogelio

RogelioMoreno · Post by **RogelioMoreno** » Sat Feb 04, 2012 12:26 pm

Charles Krebs wrote:Rogelio,

These are great!

Very talented user, great equipment and set-up.

More than any of the images you have posted recently I think these also show what I had wondered about earlier... the benefit of having gravity working for you instead of against you. With an inverted microscope you have situations where, due to gravity, multiple key parts of the subject are right up against the coverslip. (This appears to be really obvious in your third image). With an upright you would be lucky to have one or two of the tentacles... 1) in the same focal plane, and 2) against the cover slip for best optical quality. Using wet mounts in an upright, gravity tends to pull your subject away from the best location for optical quality. Often you will have a considerable layer of water between the cover and the subject... and this can really kill the image quality of a high NA objective due to increased spherical aberration. (With live material I think this can also lead to more "flattened" and distorted subjects when the photomicrographer tries to minimize this water layer).

Most inverted microscopes I have seen are set-up with very modest (NA) condensers and no DIC capability, so it is quite a revelation to see your wonderful images.

Charles,

Excellent observation, now we know other advantage of the inverted scope.

Thank you very much for your comments.

Rogelio

arturoag75 · Post by **arturoag75** » Sat Feb 04, 2012 2:59 pm

Many thanks for explanation!

arturo

Jean-marc · Post by **Jean-marc** » Sun Feb 05, 2012 9:33 am

Hi

Amazing photo !
TY for your explanation.

Jean-Marc

RogelioMoreno · Post by **RogelioMoreno** » Sun Feb 05, 2012 10:08 am

Arturo, you are welcome.

Jean-Marc, thank you. You are welcome.

Rogelio