Vorticella - Nikon DIC at maximum extinction

RogelioMoreno · Post by **RogelioMoreno** » Sun Jan 01, 2012 8:10 am

I was testing my Nikon DIC system (on the TE300) at maximum extinction position of the the Senarmont polarizer.

Here two sample pictures of vorticella at maximum extinction:

On the following link you can see a video that shows the range contrast:

http://www.youtube.com/watch?v=rLDr018KQKo

Happy 2012

Rogelio

Mitch640 · Post by **Mitch640** » Sun Jan 01, 2012 10:43 am

Nice stills and the video was very informative. I had no idea you could adjust the darkfield portion of the image.

Walter Piorkowski · Post by **Walter Piorkowski** » Sun Jan 01, 2012 3:00 pm

I enjoyed your video very much and was surprized how nicely it displayed the moving cilia. Walt

RogelioMoreno · Post by **RogelioMoreno** » Mon Jan 02, 2012 7:48 am

Mitch,

It was a like DF using DIC (not real DF). Thank you for your comments.

Walter, thank you.

Rogelio

Mitch640 · Post by **Mitch640** » Mon Jan 02, 2012 9:03 am

Ah, OK, I was confused. I meant in the first post, it was like using a polarizer at maximum extinction, not darkfield. If you remember that polarizer you told me about on E-bay? That one will go full black also, and I know DIC is similar Polarized light.

RogelioMoreno · Post by **RogelioMoreno** » Mon Jan 02, 2012 9:29 am

Mitch,

DIC is based on polarized light, a DIC system use a polarizer, analyzer and two specials prisms. Some DIC system gives black background at maximum extinction. 10x objective does not give black background.

Rogelio