again a tintinnid ciliate

Images made through a microscope. All subject types.

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Franz Neidl
Posts: 747
Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

again a tintinnid ciliate

Post by Franz Neidl »

Currently I have many tintinnids in my samples. This is a ciliate with a lorica which is 100 µm high. Somebody knows the name of the species?

Franz

Image

jc maccagno
Posts: 509
Joined: Tue Dec 21, 2010 5:43 pm

Tinninitid

Post by jc maccagno »

Hello Franz,

Can't help you with ID..what magnification and what type of lens pleaes...a lovely picture

john

Franz Neidl
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Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

Post by Franz Neidl »

Thank you John! I used a 40x PlanApo Objectiv in DIC, the PLI 2x projection eyepiece from Nikon and an electronic flash.

Franz

Mitch640
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Post by Mitch640 »

Wow Franz, a beautiful image. Was he free swimming, lorica and all? :)

BJ
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Joined: Sat Sep 29, 2007 10:53 am
Location: England

Post by BJ »

Franz,

another fantastic shot. I have no idea of the species. I love to see live tintinnids, but without a flash it is frustrating trying to get good photos!

thank you!

Brian

Charles Krebs
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Post by Charles Krebs »

Franz,

Another fascinating specimen, nicely photographed.
You certainly have an ability to get interesting subjects on your microscope. Could you give a brief summary about how you work once you have collected with the plankton net? How do you isolate and separate these subjects and get them on a slide? Is it simply done with a stereo microscope and pipettes?

Franz Neidl
Posts: 747
Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

Post by Franz Neidl »

"Could you give a brief summary about how you work once you have collected with the plankton net? How do you isolate and separate these subjects and get them on a slide? Is it simply done with a stereo microscope and pipettes?"
Hello Charles,

thank you very much for your kind words. I dont use the classical method with the stereo microscope, but I make at home a concentration from each sample with a cup-shaped planktonnet (meshsize 33 µm). Afterwards I give with a pipette two big drops on a slide and observe it on my microscope with the 4xObjective (this objective is essential for my work). If I find something interesting I cover the 2 drops with one coverglass (24x60 mm).
If I dont find something I clean the the slide and give two new drops on the slide...and so one.
If somebody wants I can describe better some of the details of this workflow.

Franz

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