Arcella sp. (testate amoeba)

Images made through a microscope. All subject types.

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NikonUser
Posts: 2688
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Arcella sp. (testate amoeba)

Post by NikonUser »

Almost 1 year ago (26 Dec 2010) I posted this image; seems like these guys like cold water:
http://www.photomacrography.net/forum/v ... 5ccd10bb4e
of a multi-nucleus Arcella sp ID'd and commented on by Ferry (thanks again).
I think I can see 10 nuclei in this one, images at 3 levels.
Olympus 40x SPlan Apo + 1.25x + 2.5x, DIC; ZS DMap stacks of 1µ frames


Image
NU11155
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Nice images. I am curious though. Was he alive and moving when you got these shots, and could you actually see the nuclei as you were watching him, or only after seeing the images?

I have yet to see a nuclei in anything as I watch them. They seem to elude me. :)

NikonUser
Posts: 2688
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Alive but fortunately not moving; the blue background caused by the DIC setup.
As to seeing nuclei: these images were sharpened but images seen through the eyepieces are always as clear/sharp as the final posted images, in fact I believe I see more detail through the scope.
DIC is really great for protozoa.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Yeah, I see the same thing, without DIC. The eyepiece view is always much sharper than anything I can get from the camera, which I haven't figured out yet apparently.

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