orange copepod (2 images added) [with universal filter]

Litonotus · Post by **Litonotus** » Sun Dec 04, 2011 12:31 pm

all that lives in that sample is red or orange...

10x Plan objective, 3 image stack, universal filter

20x achromat objective, 4 image stack, universal filter

Jan l'Amie · Post by **Jan l'Amie** » Sun Dec 04, 2011 1:05 pm

Great pictures.

curt0909 · Post by **curt0909** » Sun Dec 04, 2011 5:30 pm

Excellent images, especially the 20x. That filter is serving you well

Franz Neidl · Post by **Franz Neidl** » Mon Dec 05, 2011 4:23 am

Hello Litonotus,

interesting pictures! I have the suspect that the color is coming from oil droplets. cfr. http://www.physorg.com/news/2011-06-dis ... eight.html , also: http://www.sciencepoles.org/news/news_d ... at_depths/

Franz

Litonotus · Post by **Litonotus** » Mon Dec 05, 2011 5:26 am

thank you all

Franz, thanks for an interesting arrticle (:

Litonotus · Post by **Litonotus** » Tue Dec 06, 2011 10:52 am

two images of a copepod, that one had not so many orange oil droplets.

10x Plan objective, universal filter

20x objective, universal filter

Will Milne · Post by **Will Milne** » Tue Dec 06, 2011 5:18 pm

Fascinating and very nice images - I'm curious what level of cropping you are doing? Would it be possible to post the uncropped versions of the images.

The reason I ask is that I have playing with your Universal filter concept and have been getting some ok results but nothing like the even backgrounds , you display , across the whole of the uncropped frame and am thinking I am doing something wrong.

Will

Litonotus · Post by **Litonotus** » Tue Dec 06, 2011 11:47 pm

thank you.

As I said before - I'm taking images the simpliest and the worst way possible - I just stick camera to the eyepiece... So the field of view is very small, it is circular of course and is aobut 40% of the camera's frame... But the filter gives good background on the whole field of view when I look through the eyepiece.

show me your images and filter maybe we find out what's wrong.

phytoplankton · Post by **phytoplankton** » Wed Dec 07, 2011 11:30 am

Will,
Does your scope have Kohler illumination? My first thought is that the filter has to be located at the correct position in the light path and the condenser has to be properly focused. First, make sure you are inserting the filter into a conjugate plane in the illumination pathway (ie. at the filter slot immediately above or below the condenser aperture diaphragm). And make sure the condenser is focused on the specimen plane by adjusting it up or down. Those are my first thoughts, maybe someone here will have a better idea.

Litonotus,
Neat copepod pics.

Litonotus · Post by **Litonotus** » Wed Dec 07, 2011 1:03 pm

here are some answers:

- I have kohler illumination but I do not use it when I use the filter because closing any diaphragm (light source or condenser) reduces obligue light effect which is necessary here
- filter is placed on the filter tray (not inside like typical circular filters) under the condenser diaphragm
- every filter position is correct ---> by changing the filter position I change effects (background borghtness and contrast)