Biopsy

Tesselator · Post by **Tesselator** » Fri Dec 02, 2011 2:04 pm

These slides are about 40 years old from my Uni days.

Mitch640 · Post by **Mitch640** » Fri Dec 02, 2011 2:31 pm

Very nice crisp and clear images. I may not know what I am looking at, but know good micrography when I see it.

bromodomain · Post by **bromodomain** » Fri Dec 02, 2011 2:33 pm

Looks like a blood cloth.

Tesselator · Post by **Tesselator** » Sun Dec 04, 2011 9:50 am

I dunno what they are either actually.

They're labeled in my handwriting and dated - whoa, really dated!

It's human tissue and I think they're from a tumorous area somewhere. I didn't prepare the slides myself but were done for me - IIRC. They were needed for some equipment I was involved in designing. Anyway, I recently cleaned out an area of my studio and came across 8 boxes of 100 prepared slides I had stored away. Someone gave me an Olympus IMT-2 fully loaded (shown above) and I wanted a place to set it up next to my others. I guess I'll sell this bugger on e-pay or something as it doesn't do anything special over my Eclipse. I wonder what I can get or what I should be asking for it...

Tesselator · Post by **Tesselator** » Wed Dec 07, 2011 11:05 am

Anyone have any ideas of what an Olympus IMT-2 Inverted Mic is worth these days?

They's specifically designed for Phase Contrast with three light paths - one to the binocs, one to an OM 35mm camera port and a multi-tube port for either other binocs or another imaging device etc. There allow for two light sources - one for the P/C unit overhead and one prismed up through the objectives (I guess those are called lightfield and darkfield respectively? And when used together in some balance it's called DIC? Is that correct?

The light path can be split 50/50 between the binocs and one of the other ports or 100/0 to the binocs. The standard objectives they typically ship with the IMT-2 are:

LWD CDPlan 40 PL 0.60
LWD CDPlan 20 PL 0.40
SPlan 10 PL 0.30
SPlan 4 PL 0.13

and are pretty typical for Oly P/C (Phase Contrast) objectives as far as quality is concerned. You can see the mic or view the manual in the 4th post in this thread: http://www.photomacrography.net/forum/v ... hp?t=15282

Keep in mind I'm not thinking of selling this to anyone here - shipping would be prohibitive at roughly $600 LOL. Rather I'm only interested in assessing it's current vale for when I go to sell it here locally in Japan. Older mics are difficult to determine the value of IMO. Some sites want more than they sold for new while others see them go for less than a single objective typically goes for ($150 ~ $300). So any information would be appreciated!

Pau · Post by **Pau** » Wed Dec 07, 2011 11:21 am

Tesselator wrote: Someone gave me an Olympus IMT-2 fully loaded (shown above) ...

I can't see it

Tesselator wrote:...There allow for two light sources - one for the P/C unit overhead and one prismed up through the objectives (I guess those are called lightfield and darkfield respectively? And when used together in some balance it's called DIC? Is that correct?
...

Well, I don't know this model but the light through de objectives is likely to be used for epifluorescence (or perhaps for epi illumination like in metallurgical ones?)
If my suppositions are true it would allow for combined Phase and fluorescence
DIC is a totally different beast. There are DIC systems both for incident and transmitted ilumination and both are based in polarized light and the recombination of the shear produced by Wollaston or Nomarski prisms

Tesselator · Post by **Tesselator** » Wed Dec 07, 2011 11:28 am

Pau wrote: I can't see it

Sorry, it's here - in the 4th post in this thread: http://www.photomacrography.net/forum/v ... hp?t=15282

Well, I don't know this model but the light through de objectives is likely to be used for epifluorescence (or perhaps for epi illumination like in metallurgical ones?)
If my suppositions are true it would allow for combined Phase and fluorescence

Yep, the optional attachment is fluorescence.

DIC is a totally different beast. There are DIC systems both for incident and transmitted ilumination and both are based in polarized light and the recombination of the shear produced by Wollaston or Nomarski prisms

Oh, OK. Thanks. I guess I have to look up DIC again.

There's a polarizer on the overhead light source - two actually.

One built into the lamp housing, and a swing in filter which looks orange at some angles and green at others.

Tesselator · Post by **Tesselator** » Fri Dec 09, 2011 12:18 pm

So, no value estimations?