Bacteria - three types

Chris_M · Post by **Chris_M** » Fri Dec 02, 2011 12:09 pm

Three types of bacteria:
Spirillum
Coccus
Bacillus

Spirillum video link:
http://www.youtube.com/watch?v=Gpavfy9bIGY
(Nikon Planapo 100x / DIC / Panasonic DMC-FX60)

Cactusdave · Post by **Cactusdave** » Fri Dec 02, 2011 12:24 pm

Excellent. It's rare to see bacteria in this degree of detail with the light microscope. You have high power DIC working beautifully.

Mitch640 · Post by **Mitch640** » Fri Dec 02, 2011 12:54 pm

Nice. I was just reading recently how the Spirillum move. Seeing the video, it all made sense, although, you still can't see the micro-flagellates.

Jacek · Post by **Jacek** » Fri Dec 02, 2011 1:27 pm

world championship

Bruce Taylor · Post by **Bruce Taylor** » Fri Dec 02, 2011 2:13 pm

Krzysztof, the detail & crispness of your images is just amazing.

Marek Mis · Post by **Marek Mis** » Fri Dec 02, 2011 10:14 pm

Chris,

I can agree with the others - your skills if about DIC are simply outstanding !
I've managed to get the PZO DIC last days but I've got some difficulties with it. I was trying to use it with the old Polish MB 30 microscope but the image quality is very poor. Could you give me some advice how to adjust it ?
What is the most important. You can give the information on "pm" in Polish.

Marek

Chris_M · Post by **Chris_M** » Sat Dec 03, 2011 1:51 am

Thank you for your comments

Mitch640, You're right, bacterial flagellum is usually not visible in the light microscope due to size below the limit of resolution but for some large species (e.g.Spirillum volutans) flagellum can be seen if we use contrasting methods like phase contrast, DF or DIC. It happened to me to see (very rarely) bacterial flagellum using DF or DIC.

Marek, of course. Please check PM.
I'm very curious results with your camera.

New video, this time Spirillum in Darkfield:
http://www.youtube.com/watch?v=R99T08Fj ... re=related

arturoag75 · Post by **arturoag75** » Sat Dec 03, 2011 1:55 am

Hi,
this darkfield shot is stunning!
arturo

curt0909 · Post by **curt0909** » Sat Dec 03, 2011 5:38 am

The quality at this magnification is amazing!

Pau · Post by **Pau** » Sun Dec 04, 2011 11:21 am

Just excellent, both stills and videos.

Chris_M · Post by **Chris_M** » Sun Dec 04, 2011 12:23 pm

One more image:

(Nikon Planapo 100x / DIC / Panasonic DMC-FX60)