Images made through a microscope. All subject types.

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Wim van Egmond
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Location: Berkel en Rodenrijs, the Netherlands


Post by Wim van Egmond »

I have been to the sea to capture plankton. There was not much life in it but using the stereomicroscope I could pick up some diatoms. This is a quick sketch of a Coscinodiscus. I still have to sort out which species.

It was photographed with DIC using a Leitz 50X NPL fluotar. It works quite o.k. on my Zeiss. I made the blue background a bit darker in lightroom.



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Post by Cactusdave »

That's a big diatom. Great detail too. Which Zeiss DIC system are you using?
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Franz Neidl
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Post by Franz Neidl »

A great picture! Congratulations.
Which camera did you use? Without flash?


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Post by RogelioMoreno »


It would be nice to know about the details.

Last edited by RogelioMoreno on Mon Nov 28, 2011 6:26 pm, edited 1 time in total.

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Post by Cyclops »

Fabulous. It looks ooge!
Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope

Wim van Egmond
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Joined: Tue Aug 15, 2006 9:28 am
Location: Berkel en Rodenrijs, the Netherlands

Post by Wim van Egmond »

Thank you! Perhaps the post processing can be done slightly better. It was shot with a Nikon D3. no flash, my flash is not working properly at the moment, the illumination is not constant. For this subject it is easy enough to use a long time exposure. I have the old Zeiss dic with the plastic sliders and one of the sliders works with this Leitz objective. All my sliders have delamination (my gear has a frail constitution).

The trick of making high res pictures with high magnification is of course making the slie as flat as possible. In this case I waited until the cover slip pressed against the diatom.

I have some more images I want to post ( I made a series of plankton in 3D) but I am about to travel so it will take some time.


Jan l'Amie
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Post by Jan l'Amie »

Great picture Wim. Can't wait to see your 3D's.
Maybe there will be another presentation at the NGVM after your travelling ?
Byomic BYO500T microscope /CIOC XDS-1 invert.
Byomic ST-340 stereomicroscope
Olympus BHM Metallurgical Microscope
Canon EOS 7D Mark II / Canon MP-E65
Cognisys StackShot

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Post by arturoag75 »

Fantastic Wim :wink: !

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Post by Litonotus »

outstanding quality. I saw this diatom too, looked the same, but it was much smaller than yours.

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Post by Mitch640 »

Beautiful picture. It looks like a honey comb in the round. :)

Wim van Egmond
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Joined: Tue Aug 15, 2006 9:28 am
Location: Berkel en Rodenrijs, the Netherlands

Post by Wim van Egmond »

It was not a very big diatom but with the 50X objective it looks big, and because I made a square image it looks big on teh screen.

Litonotus, I had no time to try your filter, but I hope to have more time after my trip. I will post some more images than. There is always too many distractions. Wish I had more time.


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Post by René »

Hi Wim, nice caption of the foramen. I guess the valve is as flat as a pancake? Coscinodiscus radiatus, probably the most common marine Coscinodiscus around the Netherlands. I've seen it went down to 15 um diameter.

Best wishes, René

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