Small Crustacean custom DIC

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

curt0909
Posts: 609
Joined: Wed Oct 26, 2011 10:06 am
Location: Pittsburgh, PA

Small Crustacean custom DIC

Post by curt0909 »

A very small crustacean from a puddle just outside Pittsburgh, PA USA. I'm not sure the species.

equipment: Leica Galen III stand, Nikon bd plan 40x, 2x teleconverter, custom dic. Probably pseudo-dic at this time. I'm still making adjustments.

Videos

http://www.youtube.com/watch?v=n6EV5wzM6tI
canon 600d 3x zoom http://www.youtube.com/watch?v=_OVy1d4z ... e=youtu.be

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Pau
Site Admin
Posts: 5956
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Re: Small Crustacean custom DIC

Post by Pau »

curt0909 wrote: Probably pseudo-dic at this time.
Not sure if it is true DIC, perhaps some kind of oblique "VOILA" effect:

http://www.photomacrography.net/forum/v ... 5934#25934

http://www.photomacrography.net/forum/v ... ight=voila
Pau

Cactusdave
Posts: 1631
Joined: Tue Jun 09, 2009 12:40 pm
Location: Bromley, Kent, UK

Post by Cactusdave »

The second image is interesting, you are certainly getting lots of 'relief' detail on the subject, but you have the typical problem of (very in this case) uneven field illumination which is a characteristic of mismatched DIC combinations and oblique illumination. As Pau says it's not clear if this is true DIC or an extreme example of the form of oblique illumination that Graham Matthews called VOILA that Pau references.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

curt0909
Posts: 609
Joined: Wed Oct 26, 2011 10:06 am
Location: Pittsburgh, PA

Post by curt0909 »

Yes the gradient is pronounced in these images. Its definitely caused by the dic prisms but its greatly increased in post processing by using contrast, curves or levels adjustment. So far the system is producing 3d relief and optical sectioning. I still have to work on increasing contrast... After some research I believe this is a copepod. Does anyone know?

Edit: Its a quick fix in post to correct the gradient. Here are the updated versions

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