Paramecium under polarized light

Images made through a microscope. All subject types.

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Paramecium under polarized light

Post by NikonUser »

I wasn't going to post these as I was expecting better images but as Chris posted his excellent image of a P. bursaria inder partially polarized light ... 0ee9045367

I thought it may be worth comparing with Chris' specimen.

I tried all combinations of polarization with my set-up: Olypus simple polarizing attachment BH2-KP that includes a rotatable substage polarizer, a 1.25x intermediate tube with another polarizer (analyser), and a 530nm wave plate.

These 2 are perhaps the best, both with all components in place; in the top image the greenish background was changed to neutral grey.

Live, active fellow in too deep water; single frames. 20x + 1.25x + 2.5x, flash
I was hoping for more clarity, perhaps different colouring, of the internal bits. Cilia came out OK.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
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The Poet at the Breakfast Table.

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Post by Chris_M »

Thanks for feedback.
Your images look very good, with crisp detail.
It seems that this specimen doesn't contain to much birefringent crystals, usually seen as shining points under crossed polarizers.

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Post by Cyclops »

Very nice! I actualy prefer this type of shot for these animals!
Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope

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