I wasn't going to post these as I was expecting better images but as Chris posted his excellent image of a P. bursaria inder partially polarized light
http://www.photomacrography.net/forum/v ... 0ee9045367
I thought it may be worth comparing with Chris' specimen.
I tried all combinations of polarization with my set-up: Olypus simple polarizing attachment BH2-KP that includes a rotatable substage polarizer, a 1.25x intermediate tube with another polarizer (analyser), and a 530nm wave plate.
These 2 are perhaps the best, both with all components in place; in the top image the greenish background was changed to neutral grey.
Live, active fellow in too deep water; single frames. 20x + 1.25x + 2.5x, flash
I was hoping for more clarity, perhaps different colouring, of the internal bits. Cilia came out OK.
NU11145
Paramecium under polarized light
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Paramecium under polarized light
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives