Paramecium under polarized light

Images made through a microscope. All subject types.

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NikonUser
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Paramecium under polarized light

Post by NikonUser »

I wasn't going to post these as I was expecting better images but as Chris posted his excellent image of a P. bursaria inder partially polarized light

http://www.photomacrography.net/forum/v ... 0ee9045367

I thought it may be worth comparing with Chris' specimen.

I tried all combinations of polarization with my set-up: Olypus simple polarizing attachment BH2-KP that includes a rotatable substage polarizer, a 1.25x intermediate tube with another polarizer (analyser), and a 530nm wave plate.

These 2 are perhaps the best, both with all components in place; in the top image the greenish background was changed to neutral grey.

Live, active fellow in too deep water; single frames. 20x + 1.25x + 2.5x, flash
I was hoping for more clarity, perhaps different colouring, of the internal bits. Cilia came out OK.
Image
NU11145
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Chris_M
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Joined: Sun Apr 03, 2011 9:38 am
Location: Poland

Post by Chris_M »

Thanks for feedback.
Your images look very good, with crisp detail.
It seems that this specimen doesn't contain to much birefringent crystals, usually seen as shining points under crossed polarizers.
Chris

Cyclops
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Post by Cyclops »

Very nice! I actualy prefer this type of shot for these animals!
Canon 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope

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