Forensic Ent. 101, BF vs. DIC.

Images made through a microscope. All subject types.

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NikonUser
Posts: 2676
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Forensic Ent. 101, BF vs. DIC.

Post by NikonUser »

My banana-bait fly trap caught many flies last week and somebody laid eggs, these hatched and the larvae began eating the bait.
There were 6 species of Blow Flies in the trap and so I can't tell which species laid eggs. There was also 1 Muscid fly species.

My knowledge of forensic entomology has been gleaned from CSI shows on TV. They seem to like Blow Flies, especially their maggots.
The mouthparts differ between species and are an aid to identification.

Top: mouthparts of 2nd instar larva; brightfield, 20x obj + 2.5x relay lens; full frame
Bottom: same but with 10x obj, 1.25x intermediate lens, + 2.5x relay lens, DIC. The higher mag. from this setup negated the use of the 20x obj.

The DIC image clearly shows the large surface areas for muscle attachments, mandibles are the hooks on the right.
The regular brightfield image gives a better overall impression of the mouthparts.
Image
Image
NU11117 NU11118

EDIT: the above makes a good story. Truth is these mouthparts are from a last instar maggot of a fruit fly Drosophila, so hardly Forensics 101.
Last edited by NikonUser on Wed Oct 12, 2011 4:44 pm, edited 1 time in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Ah, very nice. I have often wished more people would post both brightfield and DIC when they post DIC. I know they are beautiful but for us DIC challenged scopers, they mean very little beyond nice looking art. ;)

Your ability to dissect an insect is phenomenal and something I'll never be able to do. It really adds to the overall effect.

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