Marine diatom

Linden.g · Post by **Linden.g** » Tue Sep 20, 2011 9:17 pm

Three images of a marine diatom. DIC flash focus stacks. The first image is the flat face and a full stack (aprox 20 frames) resulting in an image dominated by the diatoms surface detail. The second image is a partial stack so you can see inside the diatom. The third image is a edge view, again a partial stack to show internal structure.

Linden

abpho · Post by **abpho** » Tue Sep 20, 2011 10:37 pm

Very neat. Lots of good delicious sharpness.

Just think of the totally awesome pictures we are able to get these days with digital cameras and some software that any Joe can buy and use it the house. Amazing. Let me see someone do that with film. Ha!!!

Linden.g · Post by **Linden.g** » Wed Sep 21, 2011 4:49 pm

Thanks for the feedback. I'd go one step further and say that many of the members of this forum have modified equipment, including automated focus stacking and flash capability which result in image quality far beyond what is seen in mainstream academia. I've also looked at new microscope products and I haven't come across models which have these combinations of features, DIC, flash, automated focus stacking and image analysis. I'm sure you can buy these types of systems but I'm guessing they are in the 20K price range.
Linden

abpho wrote:Very neat. Lots of good delicious sharpness. Just think of the totally awesome pictures we are able to get these days with digital cameras and some software that any Joe can buy and use it the house. Amazing. Let me see someone do that with film. Ha!!!

abpho · Post by **abpho** » Wed Sep 21, 2011 4:58 pm

What is DIC? I have seen it on certain objectives but cannot find any other reference.

So, some microscopes come with flash capabilities? Or it that part of the camera that is connected to the microscope?

And what kind of image analysis? Someone was telling me that a friend of theirs (I know, one of those stories) works in a lab that has a microscope that can be programmed to run certain tests. Which they do every night. When they come back in the morning they have their results.

Mitch640 · Post by **Mitch640** » Thu Sep 22, 2011 3:51 am

Wonderful images Linden. The detail is out of this world.

RogelioMoreno · Post by **RogelioMoreno** » Thu Sep 22, 2011 4:20 am

Very nice!

Rogelio

Linden.g · Post by **Linden.g** » Sat Sep 24, 2011 6:34 am

abpho

Here is my full system with labelled modification http://www.flickr.com/photos/13084997@N ... 8697012197. The flash modifications follows Charles Krebs design, http://www.krebsmicro.com/microsetup2/index.html. Here is my version http://www.flickr.com/photos/13084997@N ... 8697012197. For focus stacking I added a StackShot drive, http://www.flickr.com/photos/13084997@N ... 8697012197. Here is a good page on DIC http://www.olympusmicro.com/primer/tech ... intro.html. Focus stacking is one step in the process of obtaining three dimensional information and you are correct in stating that a number of companies have automated diagnostic methods for tissue analysis.

All the best

Linden

abpho wrote:What is DIC? I have seen it on certain objectives but cannot find any other reference.

So, some microscopes come with flash capabilities? Or it that part of the camera that is connected to the microscope?

And what kind of image analysis? Someone was telling me that a friend of theirs (I know, one of those stories) works in a lab that has a microscope that can be programmed to run certain tests. Which they do every night. When they come back in the morning they have their results.