Hello ¡
I publish three microbes; they come from a river and reservoir between algae.
Plagiopyla nasuta 40X DIC
Loxodes 40X DIC
Brachonella spiralis 40X
Brachonella spiralis, Plagiopyla nasuta, loxodes.
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Very imprssive images. This DIC thing rules!
How do you image live organisms with 40X objective? Do you put them between coverglass and slide with little water in between? I tried putting dirty water into glass slide with cavity but image quality was much to be desired in both light and dark fields an anything but 10{ objective. Just putting them between slide and cover slip gives better picture but water evapores very quickly.
How do you image live organisms with 40X objective? Do you put them between coverglass and slide with little water in between? I tried putting dirty water into glass slide with cavity but image quality was much to be desired in both light and dark fields an anything but 10{ objective. Just putting them between slide and cover slip gives better picture but water evapores very quickly.
- Cactusdave
- Posts: 1631
- Joined: Tue Jun 09, 2009 12:40 pm
- Location: Bromley, Kent, UK
Up to your usual standard Abel, which is to say sensational. You have one of the best performing combinations of microscope, DIC system and camera that I have seen whether in professional or amateur hands. (That's meant to be a drooling smiley!)
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear
-
- Posts: 607
- Joined: Sun Oct 01, 2006 7:26 pm
- Location: NW USA
-
- Posts: 2982
- Joined: Fri Nov 20, 2009 11:24 am
- Location: Panama
Abel, extraordinary images again!
My favorite is the n3, with its comic like "face".
Because I'm in the way of set up a DIC system with mixed parts, I have some technical questions about your setup:
- What DIC prism slider do you use? U-DICT, U-DICTS... . Does it induce color casts both when it's at the right position and when it's out of it?
- What is the distance between this prism slider and the objective shoulder?
- Is there any optical element between the objective and the DIC prism?
- I'm interested in the other end of your fiber optic light guide, where it meets the flash. Do you use any kind of lens to colimate the light into the fiber optic bundle?.
My favorite is the n3, with its comic like "face".
Because I'm in the way of set up a DIC system with mixed parts, I have some technical questions about your setup:
- What DIC prism slider do you use? U-DICT, U-DICTS... . Does it induce color casts both when it's at the right position and when it's out of it?
- What is the distance between this prism slider and the objective shoulder?
- Is there any optical element between the objective and the DIC prism?
- I'm interested in the other end of your fiber optic light guide, where it meets the flash. Do you use any kind of lens to colimate the light into the fiber optic bundle?.
Pau
- Charles Krebs
- Posts: 5865
- Joined: Tue Aug 01, 2006 8:02 pm
- Location: Issaquah, WA USA
- Contact: