Brachonella spiralis, Plagiopyla nasuta, loxodes.

Images made through a microscope. All subject types.

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ABEL
Posts: 168
Joined: Wed Jul 07, 2010 5:30 am

Brachonella spiralis, Plagiopyla nasuta, loxodes.

Post by ABEL »

Hello ¡

I publish three microbes; they come from a river and reservoir between algae.

Plagiopyla nasuta 40X DIC

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Loxodes 40X DIC

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Brachonella spiralis 40X

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dennisua
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Location: Kiev, Ukraine
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Post by dennisua »

Very imprssive images. This DIC thing rules!
How do you image live organisms with 40X objective? Do you put them between coverglass and slide with little water in between? I tried putting dirty water into glass slide with cavity but image quality was much to be desired in both light and dark fields an anything but 10{ objective. Just putting them between slide and cover slip gives better picture but water evapores very quickly.

ABEL
Posts: 168
Joined: Wed Jul 07, 2010 5:30 am

Post by ABEL »

Hello.

I make water small between coverglass and slide but sufficient water in order that the microbes could swim with naturalness. I never use microscope slide with cavity.

Cactusdave
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Location: Bromley, Kent, UK

Post by Cactusdave »

Up to your usual standard Abel, which is to say sensational. You have one of the best performing combinations of microscope, DIC system and camera that I have seen whether in professional or amateur hands. =P~ (That's meant to be a drooling smiley!)
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

discomorphella
Posts: 607
Joined: Sun Oct 01, 2006 7:26 pm
Location: NW USA

Post by discomorphella »

Hi Abel--

Those are spectacular pictures (as usual). Are you using a U-CA or U-ECA magnification changer on your BX-51? Also, could you post a picture of how you bring the flash illumination into your BX illuminator?

Thanks,

David

ABEL
Posts: 168
Joined: Wed Jul 07, 2010 5:30 am

Post by ABEL »

Hello ¡

This is the adapter of the flash with the optical fiber; inside the adapter there are the lenses for Kohler's light.

Image

RogelioMoreno
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Location: Panama

Post by RogelioMoreno »

Abel,

Beautiful set!

Rogelio

Pau
Site Admin
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Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Abel, extraordinary images again!
My favorite is the n3, with its comic like "face".

Because I'm in the way of set up a DIC system with mixed parts, I have some technical questions about your setup:
- What DIC prism slider do you use? U-DICT, U-DICTS... . Does it induce color casts both when it's at the right position and when it's out of it?
- What is the distance between this prism slider and the objective shoulder?
- Is there any optical element between the objective and the DIC prism?
- I'm interested in the other end of your fiber optic light guide, where it meets the flash. Do you use any kind of lens to colimate the light into the fiber optic bundle?.
Pau

Marek Mis
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Location: Suwalki, Poland
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Post by Marek Mis »

Abel,

Perfect images, as usual. Wonderful colours and details.

Marek

Charles Krebs
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Post by Charles Krebs »

Abel,

Once again, gorgeous images!

Ecki
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Post by Ecki »

Bonjour Abel,

Wonderful pictures!

Regards
Ecki

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