ENT 101- female moth genitalia

Images made through a microscope. All subject types.

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NikonUser
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ENT 101- female moth genitalia

Post by NikonUser »

EDIT: was "What is this"
OK folks, I've been too easy on you in the past, more likely you have been too smart for me.
The gloves are off. This one is impossible!
Full frame (23.6mm wide) @ about 125x magnification.

Lots of clues:
A sphere, 1.1 mm diameter.
Smooth on outside but inside wall full of spikes.
Spike bases resemble the buttress base of the larger equatorial rain forest trees.
This is an inside view of the wall after the sphere was broken open.
I think the black jig-saw-puzzle piece is a broken cross section near a spike's tip.

Ignore the blotchines, I still can't fathom DMap.
Image
NU11100
Last edited by NikonUser on Tue Jun 14, 2011 5:43 am, edited 2 times in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

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Mitch640
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Post by Mitch640 »

I've looked at this two or three times since last night and don't have a clue. I'll wait till someone guesses. :)

NikonUser
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Location: southern New Brunswick, Canada

Post by NikonUser »

Time for the next clues.
The sphere is at the end of a tube.
Often there is a second sphere (with a tube) inside.

Outside surface showing flat bases of the spines and part of the second sphere.
About 60x on sensor.
Image
NU11100
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Planapo
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Location: Germany, in the United States of Europe

Post by Planapo »

Hmm...:-k Is that second sphere with the second tube inside the first sphere, or is it just that the two spheres and tubes are inside the thingy you have taken all these spheres and tubes out of? :?

And is the 'student of entomology' doing entomological studies here? :) :wink:

--Betty

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Hmm indeed!
yes, the second sphere with the second tube is inside the first sphere and its tube.
And, the 2 spheres and tubes are inside the thingy (a moth) that I have taken these spheres and tubes out of.

That almost gives away the answer.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

lauriek
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Post by lauriek »

I was gonna say moth testicles but one testicle inside another one makes no sense, so I really don't know, no idea at all!

However I just wanted to say I love the shots! Also what lens did you use for the first 125x shot?

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Hey Laurie, that's very good. You are on the right track, sort of 50% correct.

the lens for the 1st shot was an Oly 40x SPlan Apo, NA 0.95
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

rjlittlefield
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Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Spermatophores?

--Rik

NikonUser
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Location: southern New Brunswick, Canada

Post by NikonUser »

Let me be super strict here.
NO.
'What is' refers to the 1st image -singular.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Eric F
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Location: Sacramento, Calif.

Post by Eric F »

I think in the moth world, it would be "bursa copulatrix" (for the female...). Very nice images NU.

lauriek
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Post by lauriek »

Do moths have scrotums?

rjlittlefield
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Post by rjlittlefield »

OK, one each spermatophore?

--Rik

Planapo
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Location: Germany, in the United States of Europe

Post by Planapo »

I'm with Eric and think it is a Bursa copulatrix. The chitinous spikes have the function to open up the spermatophore which would be the second sphere with tube that is often inside, but not in your picture here.

Great photos NU, I have never seen it in such a beautiful photomicrograph. That will make it stick to my mind.

--Betty

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks everyone for playing this game, and for the kind comments. Eric got it correct.

With moths, fertilization is internal although the egg is not actually fertilized until immediately before it is laid - so, almost external fertilization.
The male has a complex intromittent organ (aedeagus, penis) for depositing sperm into the female.

The female has a relatively large pouch (the bursa copulatrix, or bursa) where the male's sperm is stored.

This is where it gets complicated.

The male's eversible aedeagus apparently extends into the female's bursa (hey guys, think of the consequences in this species).

Copulation is a lengthy process but in the end the male's sperm ends up in a relatively thick-walled sac (a spermatophore) in the bursa, and
connected to the tip of the female's abdomen by a tube:

Image
1 - bursa
2 - male's spermatophore (sperm package)
3 - duct
4 - ostium
5 - diverticulum

The size of the spermatophore suggests that it is formed inside the bursa rather than it being deposited fully formed.
This would also account for the long copulation time.

The basic structure of both male and female genitalia are consistent for all moths, however the actual 'arrangement' of the bits and pieces is unique for all species (a few exceptions) and thus examination of the genitalia is a basic tool for species identification.

These images are from a small geometric moth a Larch Pug (Eupithecia lariciata).

An image of an empty bursa copulatrix, for this species, on the MPG site:
http://mothphotographersgroup.msstate.e ... ges=7548.2
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

rjlittlefield
Site Admin
Posts: 23972
Joined: Tue Aug 01, 2006 8:34 am
Location: Richland, Washington State, USA
Contact:

Post by rjlittlefield »

Super job! I've read about the bursa copulatrix before, but nothing at the level of detail that talked about these spines. Thanks for putting this puzzle together -- that will definitely make the learning experience memorable!

--Rik

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