Cladocera

gpmatthews · Post by **gpmatthews** » Sun Jun 05, 2011 12:37 pm

Water fleas from today's catch

Eurycercus lamellatus
Microscope: Zeiss Standard GFL
Ocular: Zeiss KPL-W 10x/18
Objective: Zeiss 1.6/0.03 - 5/0.1
Darkground. Background retouched
Stack of 10 images/Zerene Stacker 1.02 Build T201008081620
Camera: Canon EOS 500D
Warnham LNR 05 Jun 2011

Daphnia (longispina?)
Microscope; Zeiss Standard GFL
Ocular: Zeiss KPL-W 10x/18
Objective: Zeiss 1.6/0.03 - 5/0.1
Darkground. Background retouched
Stack of 28 images, Zerene Stacker 1.02 Build T201008081620
Camera: Canon EOS 500D
Sample from Warnham LNR 05 Jun 2011

Charles Krebs · Post by **Charles Krebs** » Sun Jun 05, 2011 12:50 pm

Graham,

You do have a knack for finding interesting water fleas. Nice shots.

What condenser arrangement are you using for darkfield with these relatively large subjects and low power objectives? The light on the subjects looks very even. I've yet to come up with a good 2X-4X darkfield with my Olympus.

gpmatthews · Post by **gpmatthews** » Sun Jun 05, 2011 12:56 pm

Hi Charlie - the condenser is a Zeiss 0.9 NA fliptop with the top flipped out and a large wheelstop (19 mm dia patch) in the filter holder. As usual, the specimens were treated with lidocaine to immobilise them.