Cosmarium polarizatión...video

fpelectronica · Post by **fpelectronica** » Sun May 29, 2011 9:22 am

Hello
This video is only a test of the adaptation of a polarization (home) to my Nikon microscope for observing biological elements, not just minerals or crystals.
Francisco
http://youtu.be/7cZzpd3_OEU

Mitch640 · Post by **Mitch640** » Sun May 29, 2011 9:41 am

Fascinating stuff Francisco. I am really curious about the pulsing effect in the Alga. I have never seen anything move inside aglae.

fpelectronica · Post by **fpelectronica** » Sun May 29, 2011 9:50 am

Hello Mitch
Thanks for your comment
I'm no expert in these organisms but, I think, it is the initial process of formation of the "channel coupling "prior to the division of these algae
Francisco
http://youtu.be/K1cnzkgDHaI

Mitch640 · Post by **Mitch640** » Sun May 29, 2011 9:56 am

It's just so strange to see such movement in a plant.

fpelectronica · Post by **fpelectronica** » Sun May 29, 2011 10:11 am

Yes, Mitch
The world of microorganisms in a drop of water never ceases to amaze and excite
Francisco

Charles Krebs · Post by **Charles Krebs** » Tue May 31, 2011 7:47 pm

Francisco,

Very nice! Polarization (and oblique illumination) are two fairly simple and inexpensive methods to use for producing dramtic images and seeing details otherwise not visible. Too bad they are so seldom used.

Are you placing different compensators in the light path to get the various color renditions? If so, what are you using?

RogelioMoreno · Post by **RogelioMoreno** » Wed Jun 01, 2011 5:37 am

Very nice video.

I would like to know more about that home made polarizer.

Rogelio

fpelectronica · Post by **fpelectronica** » Wed Jun 01, 2011 11:15 am

Hello
Thanks Charles for your comment
Use different types of compensating filters, usually acrylic and even cellophane tape (good results).
I try with different plastic
In the video I used bright-field illumination, oblique, polarization, phase contrast, polarization & phase contrast
Thanks Rogelio
I used two pieces of polarizing filter of an LCD display (useless). It is a cheap and good quality for polarizing filters. Cost almost 0
Greetings
Francisco

Mitch640 · Post by **Mitch640** » Wed Jun 01, 2011 11:28 am

Are there different kinds of polarizers? I have a polarizer that goes over the outlet of my transmitted light on the base of the Fluophot, and the second part that goes into the slot above the nose turret. It works, but I never get colors like you are getting. So obviously, there are different kinds of polarizers?

fpelectronica · Post by **fpelectronica** » Wed Jun 01, 2011 11:44 am

Hi Mitch
No different types of polarizers
The colors "strong" are achieved by inserting a compensating filter.
You can insert a piece of acrylic or cellophane between the illumination of the base and the sample. The turn brings out beautiful colors.
Greetings
Francisco

Mitch640 · Post by **Mitch640** » Wed Jun 01, 2011 11:52 am

Thanks for the explanation Francisco. I will try it.

Mitch640 · Post by **Mitch640** » Wed Jun 01, 2011 11:59 am

HAHA, that is simply amazing. It worked very well. I will be using my polarizer much more often now.