I have 10 of these
NU11084
Caterpillar details
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Caterpillar details
EDIT: was "What am I?"
I have 10 of these
NU11084
I have 10 of these
NU11084
Last edited by NikonUser on Sun May 29, 2011 4:28 am, edited 1 time in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
50% correct
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
For you to guess 
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
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rjlittlefield
- Site Admin
- Posts: 23223
- Joined: Tue Aug 01, 2006 8:34 am
- Location: Richland, Washington State, USA
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Rik: Yes; all ZS PMax
Here's the rest of him/her:
Previous image was a crop of the next top one, 20x + 1.25x + 2.5x transmitted light
Next, abdomen showing the anterior 4 sets of prolegs, 10x on bellows, reflected light
Then Head and Thorax showing 3 pairs of true legs (note these are jointed). The 4 dots on the head are primitive eyes. 10x on bellows
Finally, entire caterpillar, length 4.0mm, 50mm El Nikkor reversed @ 1/2 stop below f/5.5
NU11088
Here's the rest of him/her:
Previous image was a crop of the next top one, 20x + 1.25x + 2.5x transmitted light
Next, abdomen showing the anterior 4 sets of prolegs, 10x on bellows, reflected light
Then Head and Thorax showing 3 pairs of true legs (note these are jointed). The 4 dots on the head are primitive eyes. 10x on bellows
Finally, entire caterpillar, length 4.0mm, 50mm El Nikkor reversed @ 1/2 stop below f/5.5
NU11088
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
-
Planapo
- Posts: 1533
- Joined: Tue Nov 07, 2006 2:33 am
- Location: Germany, in the United States of Europe
Very nice! A pleasure to look at so much details.
Hmm, obviously you didn't let him/her use the bathroom first and now amongst others, conditions at the "business end" in the last photo could indicate that he/she got a bit squeezed and already's lost a bit of what once was his/her breakfast, perhaps he/she had nibbled some yellow petals.
In short, what I really wanted to ask (as I hope to have my BHS up and running for photography soon... finally!:roll: : I figure you've used a cover slip here, now, was the specimen prepared with a chemical KOH(aq.) or lactic acid or something like that to brighten it up and give it that nice transparency? And in what liquid is the specimen embedded under the cover glass?
--Betty
Hmm, obviously you didn't let him/her use the bathroom first and now amongst others, conditions at the "business end" in the last photo could indicate that he/she got a bit squeezed and already's lost a bit of what once was his/her breakfast, perhaps he/she had nibbled some yellow petals.
In short, what I really wanted to ask
(as I hope to have my BHS up and running for photography soon... finally!:roll: : I figure you've used a cover slip here, now, was the specimen prepared with a chemical KOH(aq.) or lactic acid or something like that to brighten it up and give it that nice transparency? And in what liquid is the specimen embedded under the cover glass?--Betty
Last edited by Planapo on Sat May 28, 2011 3:32 pm, edited 1 time in total.
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rjlittlefield
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- Location: Richland, Washington State, USA
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Thanks Betty and Rik.
Betty: Preparation was the standard 5% KOH > water wash > dilute glycerine > pure glycerine.
Deep wet mount in glycerine, cover slip.
The caterpillar was not squeezed in the usual sense; it was a full 3-dimensional. What happened was that the gut was full of green leaves (Red Maple Acer rubrum). The KOH turned these to yellow and also made the caterpillar swell. When moved to water the water passed into the caterpillar and even more pressure built up inside the skin forcing the gut contents (and I think the cuticlular lining of the hind gut) out through the anus. I did remove a piece of 'this stuff' before placing on the slide but I was reluctant to remove the other piece for fear of damage and possible collapse of the caterpillar.
Rik: Yes 4.0 mm under internal pressure, in life a little bit shorter. It's a 1st instar from the batch of Eupsilia vinulenta eggs that I recently imaged.
Photographed with my BHS DIC scope but with the DIC equipment dis-engaged.
Betty: Preparation was the standard 5% KOH > water wash > dilute glycerine > pure glycerine.
Deep wet mount in glycerine, cover slip.
The caterpillar was not squeezed in the usual sense; it was a full 3-dimensional. What happened was that the gut was full of green leaves (Red Maple Acer rubrum). The KOH turned these to yellow and also made the caterpillar swell. When moved to water the water passed into the caterpillar and even more pressure built up inside the skin forcing the gut contents (and I think the cuticlular lining of the hind gut) out through the anus. I did remove a piece of 'this stuff' before placing on the slide but I was reluctant to remove the other piece for fear of damage and possible collapse of the caterpillar.
Rik: Yes 4.0 mm under internal pressure, in life a little bit shorter. It's a 1st instar from the batch of Eupsilia vinulenta eggs that I recently imaged.
Photographed with my BHS DIC scope but with the DIC equipment dis-engaged.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
Thanks Mitch.
I have been dishing out advice on how to make insect mounts in glycerine so thought it a good idea to try it out again for myself.
Killed one of my 1st instars by freezing, placed it in hot (60 C) KOH for 15 mins., rinsed in several changes of hot water, placed it in 10% aqueous glycerine soln. and heated it at 60 C until all the water had evaporated (about 5 hrs). Into fresh glycerine on a slide and photographed.
For mature insects with a harder cuticle I would normally let the insect dry completely, then place it in cold 5% KOH overnight, wash in several changes of cold water and dissect out the parts I want to photograph.
Remove as much soft tissue as possible and if necessary repeat the KOH treatment and then place the parts in 10% glycerine and leave until all the water evaporated - sometimes as long as 24 hrs.
Not necessary, and likely not good practice, to let such delicate immature insects as this caterpillar dry out before processing (they would likely shrivel).
For heating purposes I have a thermostatically controlled hot plate. NaOH works as well as KOH.
But being impatient I decided to speed up the process for this caterpillar. Turned out OK.
I have been dishing out advice on how to make insect mounts in glycerine so thought it a good idea to try it out again for myself.
Killed one of my 1st instars by freezing, placed it in hot (60 C) KOH for 15 mins., rinsed in several changes of hot water, placed it in 10% aqueous glycerine soln. and heated it at 60 C until all the water had evaporated (about 5 hrs). Into fresh glycerine on a slide and photographed.
For mature insects with a harder cuticle I would normally let the insect dry completely, then place it in cold 5% KOH overnight, wash in several changes of cold water and dissect out the parts I want to photograph.
Remove as much soft tissue as possible and if necessary repeat the KOH treatment and then place the parts in 10% glycerine and leave until all the water evaporated - sometimes as long as 24 hrs.
Not necessary, and likely not good practice, to let such delicate immature insects as this caterpillar dry out before processing (they would likely shrivel).
For heating purposes I have a thermostatically controlled hot plate. NaOH works as well as KOH.
But being impatient I decided to speed up the process for this caterpillar. Turned out OK.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
He turned out alive looking. I wonder if there is a market for insect taxidermy, or even insect funeral parlors.
My first attempt with the wasp lasted days in the processing. I should have just speeded things up I guess. I have a couple of june bugs in the freezer right now. Caught them flying around last night in my room.
My first attempt with the wasp lasted days in the processing. I should have just speeded things up I guess. I have a couple of june bugs in the freezer right now. Caught them flying around last night in my room.
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Neil Fitzgerald
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