Tardigrade (Water Bear) and link to video

Images made through a microscope. All subject types.

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Charles Krebs
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Tardigrade (Water Bear) and link to video

Post by Charles Krebs »

These are two frames pulled from a video of water bears. This part showed the specimen under crossed polarization. The two piercing stylets (used to puncture the cell wall so it can suck out the contents) are quite birefringent as are the particles in the intestine. The sucking pharynx and the muscles that control the stylets also show some birefringence as well, but are much less intense. With crossed polarizers even the 100watt halogen bulb illuminator is barely adequate, so ISO settings needed for this segment were unfortunately quite high.

I'm still playing with the video on the T3i. Below is the link to a "wmv" version. It's a large file (40 meg), but it should "stream". (Windows Explorer should do it. With Firefox you need to have the Microsoft Windows Media Player Firefox Plug-in installed.

http://krebsmicro.com/videos/tardigrade3.wmv


Image

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Last edited by Charles Krebs on Mon May 16, 2011 5:27 pm, edited 2 times in total.

fpelectronica
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Post by fpelectronica »

VERY VERY good
Francisco

RogelioMoreno
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Post by RogelioMoreno »

Excellent video.

Rogelio

Wim van Egmond
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Post by Wim van Egmond »

Super stuff, Charlie! And how did you do that zoom-in? I have always wondered why there are hardly any documentaries on television about microorganisms but I think it is because there is not enough good footage. I think this will change soon. :)

Wim

Mitch640
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Post by Mitch640 »

Wonderful video. There is so much more information in a video that a still, no matter how good it is.

Charles Krebs
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Post by Charles Krebs »

Thanks all.

Wim...
And how did you do that zoom-in?
Very simple and easy to do in the video editing software (even for someone like myself who is pretty new to video). :wink:

If anyone checks out the video I would be appreciative if you could tell me it it "streamed", or if it was necessary to download the full file ( :shock: ) before you could see it.

RogelioMoreno
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Post by RogelioMoreno »

Charles,

In my case it was necesary to download the full file before I could play it.

Rogelio

lauriek
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Post by lauriek »

Yeah Chrome just downloaded the whole thing then opened it in VLC for me... Very nice video!

Mitch640
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Post by Mitch640 »

It buffered the first 20 seconds or so, then streamed the rest without a blip. Very nice.

rjlittlefield
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Post by rjlittlefield »

Charles Krebs wrote:
And how did you do that zoom-in?
Very simple and easy to do in the video editing software (even for someone like myself who is pretty new to video). :wink:
I think I must have overlooked... Which video editing software did you use?

--Rik

Charles Krebs
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Post by Charles Krebs »

Rogelio... can you tell me which browser you use? (and is it on a PC or Mac?)

As I said, this is all pretty new to me, and I think the HD video is much nicer to watch than the old 640x480. But I sure don't want people to need to download the huge files. I know there is a procedure I need to do on my end to stream "mov" file videos to Mac users (or other Quicktime users), and I have not done that yet. But I'm not sure if there is any procedure I need to do for Windows and "wmv" files. I had the impression that it may be some setting in the viewers browser that determines whether it will stream or not.

If anyone is knowledgeable on this issue I would love to learn more about it. In my case the Windows Explorer browser that came with Windows 7 steams it "automatically" (after a little buffereing, like Mitch's experience). With Firefox I needed to add the plug-in mentioned above. Before installing that plug-in, Firefox would only do a full download.

Rik,
It is a very inexpensive program called Serif MoviePlus X5. (I use another Serif program called PagePlus that I like a great deal, and I got a "user" discount for this. Cost me about $50) I played around with a demo of Adobe Premier a few years back, but that was too overwhelming for a novice. (As was the price!). If I stick with the video I may eventually need to look at more "sophisticated" software, but this suits me for now.

RogelioMoreno
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Post by RogelioMoreno »

Charles,

Today I did try with internet explorer (windows 7, PC) and after a 100% buffereing the video started to play.

Rogelio

Cactusdave
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Post by Cactusdave »

Terrific video. I am just experimenting with interfacing a simple consumer HD camcorder to the microscope, but I have a way to got to get images anything like as good as yours.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

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