Nikon DIC First Light

RogelioMoreno · Post by **RogelioMoreno** » Wed Apr 20, 2011 10:17 pm

After about 6 months that I decided to sell all my finite Nikon microscope items to upgrade to infinity DIC, today I could take a partial DIC image with a Nikon CFI60 Plan Fluor 40x/0.75 (I say partial because the objective prism that I used for the test was the PA/PF 100 oil instead of the PF 40).

The equipment was:
- Custom modified (to accepts DIC nosepiece) Nikon E400 frame.
- Custom build Senarmont polarizer.
- Custom modified Nikon Turret Condenser for Ti (Inverted microscope), I had to modified this condenser to use it on the E400.
- Nikon DIC condenser prism 0.5 - 1.0 NA for oil lens.
- Nikon CFI60 Plan Fluor 40x/0.75
- Nikon DIC objective prism PA/PF 100 oil.
- Nikon rotatable analyzer for E400 / E600.
- Nikon infinity trinocular head - direct projection.
- Optem Optizoom at 2x - mounted between the analyzer and the trinocular head.
- Canon 50D camera.

The final image on sensor was about 80x (40 objective x 2 Optizoom).

The test slide was Lyrella lyra, single shot:

Rogelio

Charles Krebs · Post by **Charles Krebs** » Wed Apr 20, 2011 10:31 pm

Rogelio,

Sounds like a lot of work to get it all "up-and-running". But if this is an indication of the image quality you'll be getting, it was time and effort well spent! Congratulations!

RogelioMoreno · Post by **RogelioMoreno** » Wed Apr 20, 2011 10:58 pm

Charles,

Thank you for your words.

It sounds like a lot of work; but when you do not have budget to get it you have to get ebay bargains and find the way to use them.

Rogelio

Mitch640 · Post by **Mitch640** » Thu Apr 21, 2011 3:33 am

Glad to see it's working Rogelio. From the time on your post, it looks like you stayed up all night getting the first shot. And it was worth it. I'll be watching for more.

RogelioMoreno · Post by **RogelioMoreno** » Thu Apr 21, 2011 5:56 am

Mitch,

Thank you.

To be honest the picture was taken yesterday morning, before leaving to work, it took no more than 5 minutes, the test slide is a prepared slide by K. Kemp. I stayed up all night reading some chapters of the book "Fundamentals of Light Microscopy and Electronic Imaging" by Douglas Murphy, I like to read and learn how things work.

Rogelio

Jean-marc · Post by **Jean-marc** » Thu Apr 21, 2011 11:42 am

Congratulation Rogelio,

It is a very good work, and the result is very good !
It is not easy to adapt all this sort of equipment.

JM

Cactusdave · Post by **Cactusdave** » Thu Apr 21, 2011 1:10 pm

Excellent work. That looks really high quality DIC. Did you convert to monochrome because of any chromatic aberration issues or simply because the subject is enhanced by it? I would not have been confident that these components would work together at all. DIC has a reputation of being very intolerant of 'mix and match' setups.

RogelioMoreno · Post by **RogelioMoreno** » Thu Apr 21, 2011 6:51 pm

Jean-marc, thank you.

Cactusdave, On monochrome the subject looks better. At he beginning the components did not work then I switched the nosepiece from back-face to front-face and the components worked.

Rogelio

Cactusdave · Post by **Cactusdave** » Fri Apr 22, 2011 12:16 am

That must have been an anxious moment. I'm so glad it worked for you in the end. It would be nice to see a photo of your set up with the various components labelled at some point, as it's hard to picture, and it sounds like a really innovative piece of work.

RogelioMoreno · Post by **RogelioMoreno** » Sat Apr 23, 2011 12:42 pm

Cactusdave,

I posted the picturesof the setup:

http://www.photomacrography.net/forum/v ... hp?t=13005

Rogelio