Fern (Nephrolepis) stem section

NileRed · Post by **NileRed** » Mon Apr 18, 2011 11:45 am

Hello,

the following pictures are an autofluorescence (blue light excitation, green barrier filter) of fern-Nephrolepis (the popular room fern) handmade stem thin section.

First picture data: Panorama of 4 pictures (Photoshop CS4), Obj Carl Zeiss Jena 6,3x Apo, iso 200, exposition time 15-20 sec, all 4 starting pictures were stacks of 10 pictures (Zerene).

Second picture was just L and b inverted channels of the first one.

Third picture data: Obj Carl Zeiss Jena 16x Apo, iso 200, exposition time 15-20 sec, stack of two pictures (Zerene).

The pictures were obtained using simple equipment shown in my previous post (Geotrichum Candidium), but without any condenser.

arturoag75 · Post by **arturoag75** » Tue Apr 19, 2011 12:29 am

Lovely shots!

may i ask you what kind of camera did you used?
best
arturo

NileRed · Post by **NileRed** » Tue Apr 19, 2011 1:08 am

Thank you.
I used Canon 550EOS.

arturoag75 · Post by **arturoag75** » Tue Apr 19, 2011 9:40 am

many thanks

arturo

Mitch640 · Post by **Mitch640** » Tue Apr 19, 2011 1:45 pm

Beautiful images. So this is with no staining? Not sure what autofluorescence means.

Craig Gerard · Post by **Craig Gerard** » Wed Apr 20, 2011 12:16 am

Fascinating stem section, beautifully presented!

I also enjoyed reading the thread mentioned in your post, Geotrichum candidum:
http://www.photomacrography.net/forum/v ... hp?p=82115

Craig

NileRed · Post by **NileRed** » Wed Apr 20, 2011 3:27 am

Thank you for the comments.

You are right, I have used no dyes, but definitely brightness was increased.

With best wishes
Roman