A stained Amoeboid

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NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

A stained Amoeboid

Post by NikonUser »

Wet mount of 2 amoeboids fixed with formalin and stained with Malachite Green.
A rather deep mount, in water, with the top amoeboid closer to coverglass than bottom amoeboid.
Note how the inner granular cytoplasm stained but the outer hyaline cytoplasm (hyaloplasm) remained unstained.
Scale is a micrometer slide photographed with same setup.

40x S Plan Achro + 1.25x intermediate lens + 2.5x relay lens; DIC, full single frame.
Image
NU11049
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

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Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Interesting dye job. Is this the same Malachite Green used as a parasite killer in fish tanks? Did it kill the amoeba?

NikonUser
Posts: 2690
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Another pet shop formulation. This one is likely to be very efficacious, contains 11.52% formalin as well as Malachite Green. One minute drop of this stuff in a drop of pond water on a slide stops everything dead in their tracks :(
"Rid-Ich Plus"
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

I was wondering what killing them like that would do. Kind of surprised it kept itself intact though.

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