Centrifuge test

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Centrifuge test

Post by NikonUser »

Inspired by Mitch's idea of using a centrifuge to concentrate aquatic samples:
I bought a used Beckman Microfuge 12 centrifuge on e-bay for a very low price ($50.00), fortunately from Canada so shipping was reasonable.
Placed a couple of 1.5 mL tubes with green pond water in the holders and gave it a spin today by cranking the speed up to "6" and then back down to "0" (anyone know what the rpm is at "6"?).
Re-suspended one green pellet in a drop of water and took a look.
No damage seems to have been done to the organisms as there were plenty of small ciliates and some amoeboids moving around on the slide.

BHS S 40x Plan Apo 1.25x intermediate tube 2.5x relay lens, DIC; flash
Both images full frame.
Top: edge of coverslip as the water evaporated shows the accumulation of millions of single-celled algae. It was like this around the entire edge and there were still thousands of these throughout the slide.
Bottom: well fed amoeboid.
NU11047 NU11048
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Charles Krebs
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Post by Charles Krebs »

That's one really nice amoeba shot!

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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Very interesting. So the little canoes are algae? Haven't seen those yet, except the euglenas.

Spinning them down sure is a lot easier than taking a chance of finding something in a drop of water.

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