Test Nikon Fluophot - Led...video

Images made through a microscope. All subject types.

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fpelectronica
Posts: 1808
Joined: Fri Feb 19, 2010 3:56 pm
Location: España

Test Nikon Fluophot - Led...video

Post by fpelectronica »

Video test for testing an illuminator LED - Luxeon 1W - Nikon microscope adapted to a Fluophot.
I used an image with little motion-rotifer - and other more moving - ciliates -
Have employed different lighting systems, oblique, darkfield .. to determine the characteristics of LED lighting
What do you think?
Francisco

http://youtu.be/D9txNnQcqmc

Pau
Site Admin
Posts: 5696
Joined: Wed Jan 20, 2010 8:57 am
Location: Valencia, Spain

Post by Pau »

Nice video, those cilliates behave like microscopic vultures.
The illumination seems to work perfectely, very even.
Because I'm thinking in transform some microscopes to LED illumination, but I'm an absolute ignorant in electronics, can you provide images and details about your setup?
Pau

RogelioMoreno
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Joined: Fri Nov 20, 2009 11:24 am
Location: Panama

Post by RogelioMoreno »

Francisco,

I also would like to see pictures of your LED setup.

Rogelio

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Not sure what to think, Francisco. I am pretty sure your low lux CCD camera can handle the LED light OK, although the second half of the movie seemed a little darker than your normal movies do.

It sure will be cooler though, and take a lot less energy to run it. :)

Franz Neidl
Posts: 747
Joined: Wed Jan 14, 2009 11:59 am
Location: Italy

Post by Franz Neidl »

Francisco,

a very clear Video! By the way: Do you know the species of the ciliates feeding on the dying rotifer?

Franz

fpelectronica
Posts: 1808
Joined: Fri Feb 19, 2010 3:56 pm
Location: España

Post by fpelectronica »

Thanks guys for your comments.

Mitch: I have no much experience in this LED illuminator, so I have try no "over-bright" this system.

Franz: I think it could be Prorodon ciliate.

LED Illuminator is made of an 1W luxeon white led and a northbridge PC cooler.

I have used an old zeiss illuminator to put this led system inside and be able to center the led inside it.

The cooler is fixed to bulb socket with 2 components Nural (or poxipol).

Dimmer is homemade (DIY) - see schematic.

Best regards,

Francisco.

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