Dismids and diatoms
Moderators: rjlittlefield, ChrisR, Chris S., Pau
Dismids and diatoms
A few more from a marsh and pond. 100x Olympus S-Plan Achromat, flash DIC.
Linden Gledhill http://www.flickr.com/photos/13084997@N03/
Hello Linden,
Very nice image.
Do you use only plan achromats, not plan apochromats ? The details on your images are very fine. I didn't expected that the plan achromats work so good. Perchaps it depends on the rest of optics in other parts of microscope - for example in the trinocular head. I use CZJ older microscope and I'm not so satisfied of my results if about Olympus plan achromats (I use 20 X and 40 X ones).
Regards
Marek Mis
Suwalki
http://www.mismicrophoto.com/
Very nice image.
Do you use only plan achromats, not plan apochromats ? The details on your images are very fine. I didn't expected that the plan achromats work so good. Perchaps it depends on the rest of optics in other parts of microscope - for example in the trinocular head. I use CZJ older microscope and I'm not so satisfied of my results if about Olympus plan achromats (I use 20 X and 40 X ones).
Regards
Marek Mis
Suwalki
http://www.mismicrophoto.com/
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
-
twebster
- Posts: 442
- Joined: Wed Jul 26, 2006 8:02 am
- Location: Phoenix "Valley of the Sun", Arizona, USA
I love your composite images. The background is so even shaded that the image looks like a single image, not like an image made of several different images put together. Well done on the technique. 
Tom Webster
Phoenix "The Valley of the Sun", Arizona, USA
The worst day photographing dragonflies is better than the best day working!
Phoenix "The Valley of the Sun", Arizona, USA
The worst day photographing dragonflies is better than the best day working!
OK, I don't see much difference when I use DIC for green algae as compared with regular brightfield. But then I have a pretty lousy DIC system. DIC seems particularly useful for showing up structures in an otherwise clear protoplasm such as protozoa and onion cells.Mitch640 wrote:Maybe it's the colors. Both seem to have good detail, but just in different ways. It would be interesting to see the same subject under the different lighting though.
See below for a direct comparison of same subject:
DESMID HERE.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.
Nikon camera, lenses and objectives
Olympus microscope and objectives
Thanks Tom, I like the composites they remind me of text book graphics.
Marek, I have a full set if achromats but no Apos. I also have a matched set of DIC optics, take a look here http://www.flickr.com/photos/13084997@N ... 8697012197. All the above images were photostacks processed using Zerene Stacker with prior removal of chromatic aberration during RAW conversion.
Mitch, I think you see more surface relief with DIC but the advantage is much less on dense coloured organisms.
Linden
Marek, I have a full set if achromats but no Apos. I also have a matched set of DIC optics, take a look here http://www.flickr.com/photos/13084997@N ... 8697012197. All the above images were photostacks processed using Zerene Stacker with prior removal of chromatic aberration during RAW conversion.
Mitch, I think you see more surface relief with DIC but the advantage is much less on dense coloured organisms.
Linden
Linden Gledhill http://www.flickr.com/photos/13084997@N03/