Slide made with selected amoeba shells

Images made through a microscope. All subject types.

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Joined: Sat Aug 05, 2006 3:56 pm
Location: Duesseldorf, Germany

Slide made with selected amoeba shells

Post by Gerd »

When collecting water samples from moor habitats, these samples often live a very long time
on a north windowsill. But even when all desmids life ended, lots of durable, empty amoeba shells
can be found there.
One wonderful challenge is, to make some selected type slides or habitat slides,
not just for aesthetic reasons but to have a permanent slide with an overview of amoeba life forms
of this certain habitat too. Because the shells are much bigger than small diatoms,
this works well with a little patience and routine.
This is one of my first, very simple arrangements of amoeba shells.
Fixative media was a shellac-solution in Isopropanol, applied to a coverglass. When the Isopropanol is evaporated,
a very thin layer of shellac remains. The amoeba shells then were arranged under the binocular.
A short heating up to 100 degrees Celsius fasten the shells to the glass.
Now Xylene is applied for about half an hour, to expel air out of the amoeba shells.
For the final mounting i let the Xylene evaporate nearly complete. Then i put a drop of mounting media,
Caedax, or Pertex to a slide, turn it around 180 degrees an pick the coverglass.

For further information of mounting diatoms, this side is very useful : ... atoms.html



The first picture shows the arrangement with a 5 x objective,
the second image is a stack of Lesquereusia spiralis out of the arrangement.
Thanks for looking,

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Location: England

Post by BJ »


I am so impressed !!

Thank you for sharing,


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Post by Mitch640 »

Yes, very impressive. I can't imagine doing this. I have seen slides like this in images, and it's just mind boggling. Very nice work. :)

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Post by SONYNUT »

posting measurements is most helpfull :-)
Just shoot it......

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Location: Duesseldorf, Germany

Post by Gerd »

When you take a look at the SEM pictures of Lesquereusia by Ralf Meisterfeld
you can see there is a little carelessness of mine...
My camera adaption turns the taken picture by 180 degrees and i forgot to retract that during
the image processing. This one give the correct impression of the
endogenous siliceous rods the test is composed of :

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Post by archer1960 »

Amazing job. A microscopic shell collection!

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