



Leitz Ortholux microscope
4X Leitz projection eyepiece plus 1/3x relay lens
Image No.1, One of two paired superior claws.
113 images at 1 micron increments
Image cropped
Leitz UO 23X Apochromat, ULTROPAK objective
Diffused Fiber Optic Illumination
Image No.2. Middle claw used to grasp the silk strands.
Approximately 40 selected images from image number 1, 1 micron increments.
Image cropped
Leitz UO 23X Apochromat, ULTROPAK objective
Diffused Fiber Optic Illumination
Image No.3. Different angle to show both superior claws.
197 images at 1 micron increments
Image cropped
Leitz UO 23X Apochromat, ULTROPAK objective
Diffused Fiber Optic Illumination
Image No.4. End of tarsus leg segment showing all 3 claws.
85 images at 5 micron increments
Image cropped
Nikon 10X Achromat,
Diffused Fiber Optic Illumination
Canon 50D
Zerene and Photoshop processing.
All spiders have claws at the ends of their legs. Web building spiders all have three claws and use the middle claw to grasp the silk strands. Non web builders have two claws. Another single claw is sometimes also found at the tip of the palpi.
In this post I present my best results of a most challenging subject, the three tarsal claws of the spider family Agelenidea, a web builder. The small size, number of thick surrounding hairs and the almost total obscuration of the third middle claw, made clear images very difficult. I did not want to falsify the subject by removing or trimming the hairs, so I worked out different orientations and alternative methods.
I found that sectioning out portions of a deep stack would reveal, although somewhat less sharp, internal details. This method was used in image number two to reveal details of the silk grasping claw.
Walt