Difflugia corona+Difflugia corona

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NikonUser
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Difflugia corona+Difflugia corona

Post by NikonUser »

EDIT: Title changed from "Difflugia conjugating"

It's incredible enough that these protozoans can build houses but that they can design each 'door opening' so that it interlocks with another's 'door opening' is mind boggling.
The following description from
HERE
"The animal generally creeps about head-downwards,so to speak;
that is to say, with the closed end of the carapace elevated
above the surface on which it is moving. Difflugioe often exhibit
the phenomenon known as "conjugation" or "zygosis." Under these
circumstances, two Difflugioe come in contact; the mouths of the
two tests are brought together ; the two animals flow backwards
and forwards into each other's tests, with an apparently complete
incorporation; and finally they separate again, and each retires to its own test."


BHS 10x S Plan Apo 1.25x intermediate lens 2.5x relay lens
11 frames, ZS PMax

Image
NU11001
Last edited by NikonUser on Tue Jan 04, 2011 2:06 pm, edited 2 times in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

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Charles Krebs
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Post by Charles Krebs »

NU,

Wonderful find, and first rate image. I've only seen this once or twice in 6 years, and never in a setting where it was possible to get a decent shot.

Marek Mis
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Post by Marek Mis »

NU,

Beautiful image !

Marek Mis
Suwalki, Poland

NikonUser
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Post by NikonUser »

Charles & Marek, Thanks :D :D :D

I left the 'couple' on the slide; 6 hours later they decided to go for a 'walk-about'; they are now back in their aquarium:

Image
NU11002
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Very cool critters. I have yet to see anything like these, other than Arcella.

Cactusdave
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Post by Cactusdave »

Very nice picture and observation. This conjugation in Difflugia has seemingly been known about for quite a while. It is nicely illustrated on page 41 in my venerable copy of 'Microscopic Freshwater Life' by F. J. W. Plaskitt published in London in 1926. Incidentally this book has a very nice little section on the testate amoebae.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Bernd
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Post by Bernd »

NU,

beautiful images! Especially the first one.

With regard to the title of your post I would like to add a few comments.
Conjugation is only found within the ciliates (and conjugation green algae). In ciliates two cells of complementary mating types fuse for a short time and exchange genetic material, i.e. haploid gametic nuclei. Thus, conjugation clearly is a sexual process.
Despite the fact that conjugation has repeatedly been reported for testate amoebae, especially in the older literature, testate amoebae do not undergo conjugation, but copulation. As depicted in your photos, two specimen join with their pseudostomes and their cytoplasm might even fuse (plasmogamy), but not their nuclei. The exchange of genetic material has not been proven and is regarded as very unlikely. Therefore, copulation is not a sexual process. Actually, it is not clear what it is good for. In some cases, a cyst might form, but germination of the cyst has also never been reported. Some authors believe that copulating amoebae are finally doomed to die.

Best regards
Bernd

Mitch640
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Post by Mitch640 »

Actually, it is not clear what it is good for.
Maybe they are just in there playing cards, or plotting a takeover? :)

Ferry
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Post by Ferry »

Excellent picture of Difflugia corona. You were very lucky and you are of course very skilled in handling the situation! These amoebae are relatively big and most of the time within a lot of debris where they live. Super!
It could well be a cell division, where one of the amoebae is the 'parent' which built the new theca and afterwards divided.

Ferry

Ecki
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Post by Ecki »

Very nice find! The first image is stunning!

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks Dave, Bernd, Ferry, & Ecki for your comments.

I have changed the title in an attempt to cover all the possibilities.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

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