Inside an Arcella test

Images made through a microscope. All subject types.

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NikonUser
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Inside an Arcella test

Post by NikonUser »

This character had a reasonably pale test that allowed me to take a peek inside. No idea what all the vacuoles are; does appear to be a green desmid somewhere in the OOF protoplasm.
Protoplasmic connections to the test can be seen at several locations.
BHS DIC 40x S Plan, 1.25x intermediate lens, 2.5x relay lens. Flash.
Image
NUM101036
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
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Post by Mitch640 »

And this came out of your sample tank in the garage? I have not seen a testate amoeba for months in my samples. Maybe I need to spike my aquarium with some river water. :)

I like how this came out. DIC illumination is pretty versitile from the looks of it. Do you need special objectives?

NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Mitch:
Yes, my garage flat-tray aquarium is still providing lots of material. I leave the lamp on for 24 hrs, mainly to prevent the whole lot freezing.
I sucked-up what I thought was some filamentous algae yesterday and it turned out to be about 1,000 Volvox of all sizes.
DIC is incredible, my old system is good but the Olympus BX51 that ABEL uses is almost unbelievable (his recent Nematode). I can't see myself ever using anything but DIC for this aquatic stuff.
My old finite Olympus system recommends regular S Plan objectives.
The older finite Nikon system has special CF N Plan DIC objectives; I don't know if these are obligatory or whether you could use any CF N Plan.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Ecki
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Location: Cape Town, South Africa
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Post by Ecki »

This looks like Arcella vulgaris or Arcella discoides to me. You can see food vacuoles, contractile vacuoles and gas bubbles.

The discus like shape prevents Arcella from turning around with the help of its pseudopodia if the pseudostome looks into the sky. To overcome this problem Arcella species can build gas bubbles that control their buoyancy and help them to turn around in case of an accident.

fpelectronica
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Location: España

Post by fpelectronica »

Very good picture
Francisco

Ferry
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Post by Ferry »

Beautiful picture. I made some pointers to show you the way :D
Most Arcella-species have two nuclei, but this one has several, indicated by the black arrows. There are undoubtedly more, but these ones are visible.
Arcella discoides is known to have up to 65 nuclei!
You can see two clear vacuoles (V) which remove water from the body mass.
A food vacuole (FV) digests food.

Image

Ferry

NikonUser
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Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks guys, Ecki, Francisco, and Ferry.
I meant to ask Ecki which 'circles' were the parts he recognized; thanks Ferry for labeling them. I was expecting only 1 nucleus and thus was really wondering what those 'circles' you labeled as nuclei were. :oops:
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Ferry
Posts: 301
Joined: Wed Nov 18, 2009 2:41 pm
Location: Netherlands
Contact:

Post by Ferry »

This is what Ecki means: an Arcella filled with gas bubbles which make him float. A quick way to escape bad circumstances or to turn his theca. He can produce this bubbles very fast.

Image

Ferry

NikonUser
Posts: 2694
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks Ferry, a nice image.
I currently have lots of these amoebas in my samples but have yet to see one with air bubbles.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

I have seen them with air bubbles in them, but of course, I thought they were holes in the shell. I actually watched them form in one Arcella, and it happened very fast. It looked very similar to this last image posted, with 5 bubbles which formed in about one second. It was very fast and I thought it was the shell 'burning' from chemicals or something in the water. :oops:

Ferry
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Post by Ferry »

The next question should be: what kind of gas is it? Oxygen of CO2 or something else? I don't know.

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