Franz,
Not this time. I had a few "clumps" of debris on this slide that kept the coverslip elevated enough to provide plenty of maneuvering room for this amoeba.
If the intended subject is very small and it is obvious the 60X or 100X will be used, I try to avoid any such debris, so that as the water evaporates there is a time "window" where the subject is close to the coverslip, but not flattened or distorted. But sometimes I'll just take a dropper and gather a bit of whatever is floating on top of my jar and put it on the slide
. I know that I'll miss many subjects because they will be too deep and resting on the slide itself. But sometimes this works OK because some subjects are at the edge of the debris up next to the coverslip. That's what happened here.
The petroleum jelly dabs in the corners method is used mostly for subjects that are fairly thick and would otherwise be crushed by the coverslip.
