Conjugating paramecia

Images made through a microscope. All subject types.

Moderators: rjlittlefield, ChrisR, Chris S., Pau

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Conjugating paramecia

Post by Charles Krebs »

Olympus BHS. Olympus 20/0.70 S Plan Apo. Olympus 1.67X NFK. Canon 50D. DIC with electronic flash.
Image

One of the best brief descriptions of this process is on one of Wim van Egmond's pages on Molecular Expressions :
During conjugation, two paramecia of compatible mating strains join together along their oral sides and a breakdown of their membranes facilitates the temporary formation of a shared cytoplasmic bridge. The micronuclei of both ciliates undergo several divisions and one from each travels across the bridge, where it fuses with a micronucleus of the opposing party. All other macro- and micronuclei then dissipate, leaving a single nucleus in both cells. Subsequently, the conjugating ciliates pull apart and their zygotic nuclei divide repeatedly to create both types of nuclei. This development is often immediately followed by the creation of new cells via fission.

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Excellent picture. I really like how the cilia are detailed in the DIC images. Are you actually able to watch this view through the eyepieces or is this only a trinocular view?

Charles Krebs
Posts: 5865
Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
Contact:

Post by Charles Krebs »

Mitch,
Are you actually able to watch this view through the eyepieces or is this only a trinocular view?
The main thing you do not "see" through the eyepieces is the cilia detail. With living subjects the cilia is usually moving rapidly, and to the eye it appears as a blur. It is the short duration electronic flash that "stops" the motion and gives the nice detail in photographs.

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Ah, I should have known that.

Post Reply Previous topicNext topic