Stentor - Peristomal Disk

Images made through a microscope. All subject types.

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NikonUser
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Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Stentor - Peristomal Disk

Post by NikonUser »

I'm learning lots of new words.
Image shows the AZM (adoral zone of membranelles); see what I mean.
Note also a few of the many nuclei (on the left); contractile vacuole (I think) on bottom right.
Colour is reasonable; it was a bluish/green.
Olympus BHS DIC, 20x S Plan Achro, 1.25x intermediate lens, 2.5x NFK relay lens; single frame, flash.
Image
NUM10121
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

NikonUser
Posts: 2694
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Another view, same setup as earlier but with 10x S Plan Achro.
Note the large number of nuclei on the left and lower part of body.
Image
NUM10122
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Pau
Site Admin
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Location: Valencia, Spain

Post by Pau »

Wow! you are getting excellent DIC images. I like in special the second one.

:smt017 with this big ear it should have a good sense of hearing :lol:
Pau

Cactusdave
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Location: Bromley, Kent, UK

Post by Cactusdave »

Some more super pictures of Stentor. I'm so envious of the quality of your pictures and Charles'.
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

Charles Krebs
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Post by Charles Krebs »

Excellent shots NU. Good color rendition for these stentors. (On second look the ones I posted a couple days ago have way too much cyan in them)

NikonUser
Posts: 2694
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

Thanks Pau, Dave, & Charles.
Looking at F/W organisms and rotating the DIC analyser shows such amazing detail that it raises microscopy to a new level of enjoyment.
Can't see me looking at F/W life forms any way other than DIC.

It must be truly amazing to be using Olympus's new DIC scopes; somewhere I saw they have 3 analyzers for specimens of different thickness.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

Marek Mis
Posts: 2587
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

NU,

The second one is simply wonderful. Amazing details and composition. Congratulations !!!

Marek Mis
Suwalki, Poland

Marek Mis
Posts: 2587
Joined: Sat Jul 10, 2010 9:56 am
Location: Suwalki, Poland
Contact:

Post by Marek Mis »

NU,

I've forgotten to add that the peristom (?) reminds me the ear :)

Marek

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

There seems to be a lot of detail in these images. How does it compare to the regular brightfield view? Did you get images both ways, or is it too much of a different setup? Just wondered, cause I never get to see them like this.

NikonUser
Posts: 2694
Joined: Thu Sep 04, 2008 2:03 am
Location: southern New Brunswick, Canada

Post by NikonUser »

DIC makes different structures show up really well. A subject that looks structureless under regular BF will show all sorts of stuff inside the cell with DIC. You would see very little of the detail I show with regular BF.
Very simple to use, just a part below the condenser and a part below the trinocular.
Unfortunately these 2 parts are very expensive, even when bought as used.
They very rarely show up on ebay where I got mine.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

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