Mallomonas insignis is a synurophyte alga. It is related to the chysophytes (like Dinobryon) but it is enclosed in a layer of siliceous scales. Very obvious are the brown/tan colored chlorophyll containing plastids.
I collected a fresh sample from my favorite (ice covered!) pond and found this species for the first time. It can look different depending on where the microscope is focused. The "inside" looks very much like a diatom. Around the cell is a "coat" of scales that reminded me of certain scaled amoeba tests. There was a single flagellum up front (not seen in these photos) and at the rear, a "tail" of scales. Interesting specimen.
In the last shot of these three I tried to get a close look at the scale "coat", and as you can see from the scale bar, we're dealing with some pretty small structures. Two "focus selections" are given to give a sense of the construction.
Mallomonas insignis
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- Charles Krebs
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Wow, some really neat images there! I got a long way to go before I can get them this good!
What objective/eyepiece combo were you using?
What objective/eyepiece combo were you using?
Canon 5D and 30D | Canon IXUS 265HS | Cosina 100mm f3.5 macro | EF 75-300 f4.5-5.6 USM III | EF 50 f1.8 II | Slik 88 tripod | Apex Practicioner monocular microscope
Quite beautiful there Charlie , don't think I have ever ran across one of these. The cold weather undoubtedly will bring about some very interesting pond critters along with diatoms and various algae, since more sunlight is getting to the water now and the DO (dissolved oxygen) levels are coming up. Though most pond critters do not need a high oxygen content, they seem to be most prolific during the cooler or colder season.
- rjlittlefield
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- Bruce Williams
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- Bruce Williams
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- Charles Krebs
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Bruce... I did take a few with oblique brightfield. Unfortunately there was no A:B comparison... so don't put too much weight on this, but this might give you some idea compared to the 4-panel shot above (although I think the "situation" for the 4-panel images was better).
The scales on the subject are clear as glass, but oblique brightfield did a good job. Now that I have used DIC for about 8 months, I must say that my previous opinion that oblique brightfield is vastly underused and underrated is only reinforced. I would never say DIC isn't wonderful, but with some practice, oblique (and some other techniques like COL) can be used very effectively in many situations where DIC works well.
Walt... Thanks!
Rik... it is a little difficult to visualize. Maybe I should go through all the shots to look for a better progression. I tried a 2 or 3 shot "stack" but wow, was that ever confusing! My impression was that is consisted of (at least) two interleaved layers of (roughly) diamond shaped scales. The first high mag image (upper) shows the layout pretty well. The second does get confusing since they are essentially clear and the edges are staggered and overlap.
The scales on the subject are clear as glass, but oblique brightfield did a good job. Now that I have used DIC for about 8 months, I must say that my previous opinion that oblique brightfield is vastly underused and underrated is only reinforced. I would never say DIC isn't wonderful, but with some practice, oblique (and some other techniques like COL) can be used very effectively in many situations where DIC works well.
Walt... Thanks!
Rik... it is a little difficult to visualize. Maybe I should go through all the shots to look for a better progression. I tried a 2 or 3 shot "stack" but wow, was that ever confusing! My impression was that is consisted of (at least) two interleaved layers of (roughly) diamond shaped scales. The first high mag image (upper) shows the layout pretty well. The second does get confusing since they are essentially clear and the edges are staggered and overlap.
- Bruce Williams
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Thanks for posting the oblique brightfield image Charlie. I have to say it stacks up surprisingly well against the DIC images, particularly considering your comment about the relative shooting conditions. However I do take your point re. caution over placing too much relevance as not actually a true A:B comparison.
Anyway your results have encouraged me to read up and experiment with OB and I hope to be posting my first images fairly soon.
Bruce
Anyway your results have encouraged me to read up and experiment with OB and I hope to be posting my first images fairly soon.
Bruce