Paramecium

Images made through a microscope. All subject types.

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ABEL
Posts: 168
Joined: Wed Jul 07, 2010 5:30 am

Paramecium

Post by ABEL »

Hello.

They are paramecium, possibly putrinum or aurelia. The sample comes from water suspended with rotten leaves.

DIC 40x, Olympus BX 51, camera Jenoptik flash.

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With possibly brachonella spiralis

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Image

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Charles Krebs
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Joined: Tue Aug 01, 2006 8:02 pm
Location: Issaquah, WA USA
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Post by Charles Krebs »

Ahhhh... simply wonderful images!

Tom Jones
Posts: 326
Joined: Sat Jan 31, 2009 2:05 am
Location: Crestline, CA

Post by Tom Jones »

Hi Abel,

Just beautiful! :D

A question about your background color. How do you get the very dark grey? Do you use the UDCB condenser, or the UDC8 condenser with the lambda plate on your BX-51, or are you doing it in post processing? A search indicates some folks, including Charlie, do it in post at least part of the time, but I'm not familiar with their DIC setups and I know there are differences. Some have retarders and some don't.

I have a BX-50 and the older DICT slider and UDCB condenser without the lambda plate and can't get beyond light blue without post processing. I have some polariziing and full wave retarder film on order from Edmund Optics to make a compensator (polarizer, full wave retarder, Nomarski prism, bottom to top), to bypass the straight polarizer on my condenser. That's what I understand the UDC8 condenser has. I'm getting quarter and half wave film to play with too. 8)

The manual for the UCD8 indicates I should be able to get grey without the lambda (full wave) plate but it just isn't happening with the UCDB.

Most of the time I prefer the light grey to dark grey background, but I can't get it directly. Am I on the right track adding the retarder or am I as clueless as I fear? :oops:

Every time I try to understand the finer points of optical physics pertaining to polarization, retardation and DIC, my poor eyes glaze over... :shock: :shock:

Thanks.

Tom

Mitch640
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Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Beautiful images Abel. I can barely follow what Tom said, but I am interested in what camera your using. :)

ABEL
Posts: 168
Joined: Wed Jul 07, 2010 5:30 am

Post by ABEL »

Hello.

I work with the universal condenser UCD8 and the prism U-DICT.

Very easy; Only, I use the prism translation control of the U-DICT and I make it turn up to obtaining the wished effect :roll: .

Tom Jones
Posts: 326
Joined: Sat Jan 31, 2009 2:05 am
Location: Crestline, CA

Post by Tom Jones »

Thanks Abel! That means I'm on the right track in adding the lambda plate to my system.

Tom

arturoag75
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Location: italy
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Post by arturoag75 »

ABEL,
i have no words..fantastic shots!
best
art

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