FW Sponge cells and spicules

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NikonUser
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Location: southern New Brunswick, Canada

FW Sponge cells and spicules

Post by NikonUser »

EDIT: was "What is it? Quiz"
All from the same organism.
The larger green cells are about 12µ in diameter.
Composite plates of single frames; the whole slide was 'buzzing' for want of a better description.
100x + 1.25x + 2.5x for about a 300x magnification.
Image
Image
NUM10091 NUM10092
Last edited by NikonUser on Sun Oct 24, 2010 12:49 pm, edited 1 time in total.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

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Ernst Hippe
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Post by Ernst Hippe »

Is it from a sponge?

Cactusdave
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Post by Cactusdave »

Sea cucumber, Synapta?
Leitz Ortholux 1, Zeiss standard, Nikon Diaphot inverted, Canon photographic gear

René
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Post by René »

Hmm, yes, must be a sponge as I have ot come across refs to endosymbiontic algae and sea cucumbers (but who knows). I also never realised they could also have chlorophyte endosymbionts. Likely a freshwater sponge then (hurrah for wikipedia). Another nice point from wiki: the silica spicules transmit light into the lumen where the endosymbiont algae live. I wonder whether that is the biggest reason for their existence?

Rene.

NikonUser
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Post by NikonUser »

Yes, a freshwater sponge from a local lake. It didn’t long for Ernst to correctly ID it.
The green, of course, is a symbiotic zoochlorellae algae in the cells of the sponge.
The spines and anchors are siliceous spicules composed of pure silica (my text recommends boiling nitric acid to remove all other material and leave just the spicules – I guess that would work!).
These spicules form the skeleton of the sponge and possibly play some part in defense; apparently snails don’t like eating sponges because of the needle-like spicules.
NU.
student of entomology
Quote – Holmes on ‘Entomology’
” I suppose you are an entomologist ? “
” Not quite so ambitious as that, sir. I should like to put my eyes on the individual entitled to that name.
No man can be truly called an entomologist,
sir; the subject is too vast for any single human intelligence to grasp.”
Oliver Wendell Holmes, Sr
The Poet at the Breakfast Table.

Nikon camera, lenses and objectives
Olympus microscope and objectives

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