Loxodes ciliate....video

[fpelectronica]

Hello

Ciliado Loxodes
El extremo anterior lo tiene curvado a modo de pico
Posee el lado izquierdo plano, sin cilios
Tamaño 150-250um
Se alimenta de algas y bacterias del azufre
Microscope Nikon
Francisco



http://www.youtube.com/watch?v=FWlCBnu0uOI

[Mitch640]

I always like watching your videos Francisco. Impressive video quality, and the colors are great. Very nice.

[john sp.]

Hi Francisco, I too am a fan of your videos. I was wondering if you could tell me a bit about the different kinds of lighting techniques that are being used. It looks like it starts out with Brightfield, but there is a shift to something else around 0:38. Is this DIC or maybe oblique lighting? Then, around 0:56, there is another kind of lighting. It almost looks like dark field or a kind of dark, phase contrast, perhaps? Then, maybe the pattern repeats, i.e., Brightfield, Oblique, and Dark Field.

I also found myself wondering what the other large ciliate is that makes its appearance in the beginning and in the ending of the video. I get the impression that it is rotating slowly as it swims and that it therefore has a circular cross-section (i.e., not flattened). I couldn't really make out a adoral groove. My best guess would be Nassula, but I was wondering if anyone else had another suggestion for what it might be.

(It looks like a Vorticella is also in there, mostly in the middle part of the video.)

Thanks for sharing this.

John

[fpelectronica]

Hello John
Thanks for your comments and for appreciating my videos
All lighting techniques that I use, are produced by the lateral movement of phase-contrast ring. The intent of these forms of illumination is to try to highlight details of the microorganisms.
I do not have - unfortunately - DIC system
I think the other ciliate could be - with complete safety - a Pseudoprorodon. I hope algien expert can tell us more
Francisco
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[john sp.]

Francisco, thanks for the reply. Since all my microscope offers to work with is brightfield, I'll have to rely on what I have read about phase contrast to understand what you described as your method for varying the lighting. I'm going to guess that this mixes some oblique lighting effects (which can simulate the appearance of DIC) into the phase contrast illumination. In any case, what you are doing is very effective at displaying the creature from different perspectives.

I liked your suggestion that the other protozoa (making a guest appearance in your video) might be Pseudoprorodon. My google of this name led to a video you had done of this creature earlier, which again shows the cylindrical body with its gentle rotation. ( http://www.photomacrography.net/forum/v ... 64d127c508 ) I particularly liked the darkfield-like part of that video, which really shows off the creature three dimensionally.

John

[fpelectronica]

Hi John
In my microscope phase contrast rings are on a rotating turret
The ring 100X and 10X lens provides a dark field image.
If I turn the ring 1 / 4, I provide oblique illumination image. 1 / 2 rotation gives me something Iuminación oblique and darkfield .... etc
I tried different combinations of rotation and different phase contrast objectives.
'll Post a video soon thecamoebians which include different combinations
I hope you understand (the Google translator is not very effective). Sorry
Francisco