A Vorticella? With "Oblique Illumination" Lighting

Images made through a microscope. All subject types.

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Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

A Vorticella? With "Oblique Illumination" Lighting

Post by Mitch640 »

I found my first Vorticella and then this effect by accident, my thumb partially covered the light source while reaching for the stage x, y knobs. Then I remembered a thread I read here about making shaped diffusers to get a similar effect. I eventually just cut a V notch out of a post-it note and laid it over the bottom light source. As usual, it looked much better through the oculars than I seem to be able to photograph.

Here is a video of him and some other critters in the water.

This is the Vorticella with 10x objective, using Oblique Illumination lighting to highlight the internals of the Vorticelli.
Image

Here he is at 40x...
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And another...
Image
Last edited by Mitch640 on Tue Oct 19, 2010 1:29 pm, edited 3 times in total.

rjlittlefield
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Post by rjlittlefield »

Nice images. This type of lighting is called "oblique illumination". As noted HERE, one of the world's best photomicrographers is on record as saying that it is an underrated technique.

--Rik

Mitch640
Posts: 2137
Joined: Sun Aug 15, 2010 1:43 pm

Post by Mitch640 »

Thanks Rik, that's it, I changed the title. I searched all over for that thread for the proper name, and the one that shows how to do it, but couldn't find it. It won't be underrated by me anymore. I'll be using it a lot. :)

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